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Literature summary for 1.14.11.55 extracted from

  • Bursy, J.; Kuhlmann, A.U.; Pittelkow, M.; Hartmann, H.; Jebbar, M.; Pierik, A.J.; Bremer, E.
    Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses (2008), Appl. Environ. Microbiol., 74, 7286-7296.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ stimulation at 1 mM, inhibitory above Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
ectoine pH not specified in the publication, temperature not specified in the publication Streptomyces coelicolor
6.2
-
2-oxoglutarate pH not specified in the publication, temperature not specified in the publication Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulation at 1 mM, inhibitory above Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32700
-
-
Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
pH not specified in the publication, temperature not specified in the publication Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ectoine + 2-oxoglutarate + O2
-
Streptomyces coelicolor 5-hydroxyectoine + succinate + CO2
-
?
ectoine + 2-oxoglutarate + O2
-
Streptomyces coelicolor A3(2) 5-hydroxyectoine + succinate + CO2
-
?
additional information no substrate: L-proline Streptomyces coelicolor ?
-
?
additional information no substrate: L-proline Streptomyces coelicolor A3(2) ?
-
?

Subunits

Subunits Comment Organism
? x * 38500, SDS-PAGE, x * 32833, calculated Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Streptomyces coelicolor

General Information

General Information Comment Organism
physiological function the accumulation of the compatible solutes ectoine and 5-hydroxyectoine allows Streptomyces coelicolor A3(2) to fend off the detrimental effects of both high salinity and high temperature on cell physiology Streptomyces coelicolor