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Literature summary for extracted from

  • Bursy, J.; Pierik, A.J.; Pica, N.; Bremer, E.
    Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase (2007), J. Biol. Chem., 282, 31147-31155.
    View publication on PubMed


Inhibitors Comment Organism Structure
ascorbate 5 mM, 70% inhibition Virgibacillus salexigens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
ectoine pH 7.5, 32°C Virgibacillus salexigens
2-oxoglutarate pH 7.5, 32°C Virgibacillus salexigens


Metals/Ions Comment Organism Structure
Fe2+ presence of Fe2+ is required Virgibacillus salexigens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
Virgibacillus salexigens
Virgibacillus salexigens
gel filtration Virgibacillus salexigens


Organism UniProt Comment Textmining
Virgibacillus salexigens Q2TDY4

Purification (Commentary)

Purification (Comment) Organism
Virgibacillus salexigens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
pH 7.5, 32°C Virgibacillus salexigens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ectoine + 2-oxoglutarate + O2
Virgibacillus salexigens 5-hydroxyectoine + succinate + CO2 reaction is dependent on iron(II), molecular oxygen, and 2-oxoglutarate ?
additional information the cosubstrate 2-oxoglutarate is stoichiometrically decarboxylated during the hydroxylation of the substrate ectoine. CO2 is thereby liberated from 2-oxoglutarate to form succinate. During the hydroxylation reaction, one atom of the atmospheric oxygen molecule is incorporated into succinate, whereas the other atom is incorporated into the hydroxy group formed on ectoine Virgibacillus salexigens ?


Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE, 1 * 34400, mass spectrometry Virgibacillus salexigens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
Virgibacillus salexigens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
Virgibacillus salexigens


Organism Comment Expression
Virgibacillus salexigens ectoine production is linearly correlated with the salinity of the growth medium. The formation of 5-hydroxyectoine is primarily a stationary growth phase phenomenon. The transcript levels of the ectABC and ectD genes increase as a function of salinity additional information