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Literature summary for 1.14.11.55 extracted from
- Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase (2007), J. Biol. Chem., 282, 31147-31155.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | 5 mM, 70% inhibition | Virgibacillus salexigens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
ectoine | pH 7.5, 32°C | Virgibacillus salexigens | |
| 5.2 | - |
2-oxoglutarate | pH 7.5, 32°C | Virgibacillus salexigens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | presence of Fe2+ is required | Virgibacillus salexigens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
- |
Virgibacillus salexigens |
| 34400 | - |
- |
Virgibacillus salexigens |
| 36000 | - |
gel filtration | Virgibacillus salexigens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Virgibacillus salexigens | Q2TDY4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Virgibacillus salexigens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.06 | - |
pH 7.5, 32°C | Virgibacillus salexigens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ectoine + 2-oxoglutarate + O2 | - |
Virgibacillus salexigens | 5-hydroxyectoine + succinate + CO2 | reaction is dependent on iron(II), molecular oxygen, and 2-oxoglutarate | ? | |
| additional information | the cosubstrate 2-oxoglutarate is stoichiometrically decarboxylated during the hydroxylation of the substrate ectoine. CO2 is thereby liberated from 2-oxoglutarate to form succinate. During the hydroxylation reaction, one atom of the atmospheric oxygen molecule is incorporated into succinate, whereas the other atom is incorporated into the hydroxy group formed on ectoine | Virgibacillus salexigens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE, 1 * 34400, mass spectrometry | Virgibacillus salexigens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
- |
Virgibacillus salexigens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Virgibacillus salexigens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Virgibacillus salexigens | ectoine production is linearly correlated with the salinity of the growth medium. The formation of 5-hydroxyectoine is primarily a stationary growth phase phenomenon. The transcript levels of the ectABC and ectD genes increase as a function of salinity | additional information |
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