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Literature summary for 1.14.11.55 extracted from

  • Czech, L.; Wilcken, S.; Czech, O.; Linne, U.; Brauner, J.; Smits, S.H.J.; Galinski, E.A.; Bremer, E.
    Exploiting substrate promiscuity of ectoine hydroxylase for regio- and stereoselective modification of homoectoine (2019), Front. Microbiol., 10, 2745 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.55

Application

Application Comment Organism
synthesis an Escherichia coli cell factory expressing the Pseudomonas stutzeri ectD gene from a synthetic promoter imports homoectoine via the ProU and ProP compatible solute transporters, hydroxylates it, and secretes the formed trans-5-hydroxyhomoectoine, independent from all currently known mechanosensitive channels, into the growth medium Stutzerimonas stutzeri

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling and docking of different substrates into the crystal structure. The spatial positioning of homoectoine and its hydroxylated derivative in the reaction chamber are super-imposable with that of the 5-hydroxyectoine molecule Sphingopyxis alaskensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 ectoine pH 7.5, 35°C Sphingopyxis alaskensis
2.3
-
 ectoine pH 7.5, 35°C Stutzerimonas stutzeri
2.6
-
 L-homoectoine pH 7.5, 35°C Stutzerimonas stutzeri
3.1
-
 L-homoectoine pH 7.5, 35°C Sphingopyxis alaskensis

Organism

Organism UniProt Comment Textmining
Sphingopyxis alaskensis Q1GNW5
-
-
Sphingopyxis alaskensis DSM 13593 Q1GNW5
-
-
Stutzerimonas stutzeri A4VFY4 i.e. Pseudomonas stutzeri
-
Stutzerimonas stutzeri A1501 A4VFY4 i.e. Pseudomonas stutzeri
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis 5-hydroxyectoine + succinate + CO2
-
 ?
ectoine + 2-oxoglutarate + O2
-
 Stutzerimonas stutzeri 5-hydroxyectoine + succinate + CO2
-
 ?
ectoine + 2-oxoglutarate + O2
-
 Stutzerimonas stutzeri A1501 5-hydroxyectoine + succinate + CO2
-
 ?
ectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis DSM 13593 5-hydroxyectoine + succinate + CO2
-
 ?
L-homoectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis trans-5-hydroxy-L-homoectoine + succinate + CO2
-
 ?
L-homoectoine + 2-oxoglutarate + O2
-
 Stutzerimonas stutzeri trans-5-hydroxy-L-homoectoine + succinate + CO2
-
 ?
L-homoectoine + 2-oxoglutarate + O2
-
 Stutzerimonas stutzeri A1501 trans-5-hydroxy-L-homoectoine + succinate + CO2
-
 ?
L-homoectoine + 2-oxoglutarate + O2
-
 Sphingopyxis alaskensis DSM 13593 trans-5-hydroxy-L-homoectoine + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 34140, calculated from sequence Sphingopyxis alaskensis
? x * 34180, calculated from sequence and SDS-PAGE Stutzerimonas stutzeri

pI Value

Organism Comment pI Value Maximum pI Value
Sphingopyxis alaskensis calculated from sequence
-
 5.5
Stutzerimonas stutzeri calculated from sequence
-
 5.5