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Literature summary for 1.14.11.6 extracted from
- Mechanism-based inactivation of thymine hydroxylase, an alpha-ketoglutarate-dependent dioxygenase, by 5-ethynyluracil (1993), Biochemistry, 32, 14034-14042.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-Ethynyluracil | mechanism-based inactivator | Rhodotorula glutinis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | non-heme | Rhodotorula glutinis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formyluracil + 2-oxoglutarate + O2 | Rhodotorula glutinis | - |
5-carboxyuracil + succinate + CO2 | - |
? | |
| 5-hydroxymethyluracil + 2-oxoglutarate + O2 | Rhodotorula glutinis | - |
5-formyluracil + succinate + CO2 | 5-formyluracil reacts subsequently to 5-carboxyuracil | ? | |
| thymine + 2-oxoglutarate + O2 | Rhodotorula glutinis | - |
5-hydroxymethyluracil + succinate + CO2 | 5-hydroxymethyluracil reacts subsequently to 5-formyluracil | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula glutinis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Rhodotorula glutinis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20 mM potassium phosphate pH 7.5, 100 mM glycine and 0.1 mM EDTA, stable | Rhodotorula glutinis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-ethynyluracil + 2-oxoglutarate + O2 + H2O | - |
Rhodotorula glutinis | 5-(carboxymethyl)uracil + succinate + CO2 | - |
? | |
| 5-formyluracil + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | 5-carboxyuracil + succinate + CO2 | - |
? | |
| 5-formyluracil + 2-oxoglutarate + O2 | 5-formyluracil reacts subsequently to 5-carboxyuracil | Rhodotorula glutinis | 5-carboxyuracil + succinate + CO2 | - |
? | |
| 5-hydroxymethyluracil + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | 5-formyluracil + succinate + CO2 | - |
? | |
| 5-hydroxymethyluracil + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | 5-formyluracil + succinate + CO2 | 5-formyluracil reacts subsequently to 5-carboxyuracil | ? | |
| thymine + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | 5-hydroxymethyluracil + succinate + CO2 | 5-hydroxymethyluracil reacts subsequently to 5-formyluracil | ? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.7 | - |
thymine | - |
Rhodotorula glutinis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
5-Ethynyluracil | - |
Rhodotorula glutinis | |
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