BRENDA - Enzyme Database
show all sequences of 1.14.11.60

Scopoletin 8-hydroxylase a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis

Siwinska, J.; Siatkowska, K.; Olry, A.; Grosjean, J.; Hehn, A.; Bourgaud, F.; Meharg, A.A.; Carey, M.; Lojkowska, E.; Ihnatowicz, A.; J. Exp. Bot. 69, 1735-1748 (2018)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression of S8H protein in Nicotiana benthamiana leaves; heterologous expression in Escherichia coli
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
Fe2+
concentrations above 0.05 mM significantly reduce the enzyme activity, leading to decreased fraxetin synthesis
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
scopoletin
pH 8.0, 31.5°C
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
enzyme efficiency is best in the presence of 0.05 mM Fe2+
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
scopoletin + 2-oxoglutarate + O2
Arabidopsis thaliana
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
fraxetin + succinate + CO2
-
-
?
scopoletin + 2-oxoglutarate + O2
Arabidopsis thaliana Col-0
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
fraxetin + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q9LE86
-
-
Arabidopsis thaliana Col-0
Q9LE86
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
scopoletin + 2-oxoglutarate + O2
-
748335
Arabidopsis thaliana
fraxetin + succinate + CO2
-
-
-
?
scopoletin + 2-oxoglutarate + O2
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
748335
Arabidopsis thaliana
fraxetin + succinate + CO2
-
-
-
?
scopoletin + 2-oxoglutarate + O2
-
748335
Arabidopsis thaliana Col-0
fraxetin + succinate + CO2
-
-
-
?
scopoletin + 2-oxoglutarate + O2
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
748335
Arabidopsis thaliana Col-0
fraxetin + succinate + CO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
At3g12900 protein
-
Arabidopsis thaliana
S8H
-
Arabidopsis thaliana
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
31.5
-
-
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.73
-
scopoletin
pH 8.0, 31.5°C
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of S8H protein in Nicotiana benthamiana leaves; heterologous expression in Escherichia coli
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Fe2+
concentrations above 0.05 mM significantly reduce the enzyme activity, leading to decreased fraxetin synthesis
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
scopoletin
pH 8.0, 31.5°C
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
enzyme efficiency is best in the presence of 0.05 mM Fe2+
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
scopoletin + 2-oxoglutarate + O2
Arabidopsis thaliana
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
fraxetin + succinate + CO2
-
-
?
scopoletin + 2-oxoglutarate + O2
Arabidopsis thaliana Col-0
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
fraxetin + succinate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
scopoletin + 2-oxoglutarate + O2
-
748335
Arabidopsis thaliana
fraxetin + succinate + CO2
-
-
-
?
scopoletin + 2-oxoglutarate + O2
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
748335
Arabidopsis thaliana
fraxetin + succinate + CO2
-
-
-
?
scopoletin + 2-oxoglutarate + O2
-
748335
Arabidopsis thaliana Col-0
fraxetin + succinate + CO2
-
-
-
?
scopoletin + 2-oxoglutarate + O2
the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis
748335
Arabidopsis thaliana Col-0
fraxetin + succinate + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
31.5
-
-
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.73
-
scopoletin
pH 8.0, 31.5°C
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in coumarin biosynthesis and is part of mechanisms used by plants to assimilate iron
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in coumarin biosynthesis and is part of mechanisms used by plants to assimilate iron
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
157.3
-
scopoletin
pH 8.0, 31.5°C
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
157.3
-
scopoletin
pH 8.0, 31.5°C
Arabidopsis thaliana
Other publictions for EC 1.14.11.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748335
Siwinska
Scopoletin 8-hydroxylase a no ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
J. Exp. Bot.
69
1735-1748
2018
-
-
1
-
-
-
1
1
-
1
-
2
-
7
-
-
1
-
-
1
-
-
4
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
1
-
2
-
-
-
1
-
1
-
-
4
-
1
-
-
1
1
-
-
-
-
1
1
-
1
1
748735
Rajniak
Biosynthesis of redox-active ...
Arabidopsis thaliana
Nat. Chem. Biol.
14
442-450
2018
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
748994
Tsai
Scopoletin 8-hydroxylase-medi ...
Arabidopsis thaliana
Plant Physiol.
177
194-207
2018
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-