BRENDA - Enzyme Database
show all sequences of 1.14.11.60

Scopoletin 8-hydroxylase-mediated fraxetin production is crucial for iron mobilization

Tsai, H.H.; Rodriguez-Celma, J.; Lan, P.; Wu, Y.C.; Velez-Bermudez, I.C.; Schmidt, W.; Plant Physiol. 177, 194-207 (2018)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
scopoletin + 2-oxoglutarate + O2
Arabidopsis thaliana
the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway
fraxetin + succinate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9LE86
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
scopoletin + 2-oxoglutarate + O2
-
748994
Arabidopsis thaliana
fraxetin + succinate + CO2
-
-
-
?
scopoletin + 2-oxoglutarate + O2
the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway
748994
Arabidopsis thaliana
fraxetin + succinate + CO2
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
scopoletin + 2-oxoglutarate + O2
Arabidopsis thaliana
the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway
fraxetin + succinate + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
scopoletin + 2-oxoglutarate + O2
-
748994
Arabidopsis thaliana
fraxetin + succinate + CO2
-
-
-
?
scopoletin + 2-oxoglutarate + O2
the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway
748994
Arabidopsis thaliana
fraxetin + succinate + CO2
-
-
-
?
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
the enzyme is highly induced in roots of Fe-deficient plants both at the transcript and protein levels
up
General Information
General Information
Commentary
Organism
malfunction
mutants defective in the expression of S8H show increased sensitivity to growth on pH 7.0 media supplemented with an immobile source of Fe and reduced secretion of fraxetin. Transgenic lines overexpressing S8H exhibit an opposite phenotype. Supplementing the media containing immobile Fe with fraxetin partially rescues the s8h mutants
Arabidopsis thaliana
metabolism
the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway. Fraxetin secretion is a decisive factor for calcicole-calcifuge behavior (i.e. the ability/inability to thrive on alkaline soils) of plants
Arabidopsis thaliana
physiological function
the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway. Fraxetin secretion is a decisive factor for calcicole-calcifuge behavior (i.e. the ability/inability to thrive on alkaline soils) of plants
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
mutants defective in the expression of S8H show increased sensitivity to growth on pH 7.0 media supplemented with an immobile source of Fe and reduced secretion of fraxetin. Transgenic lines overexpressing S8H exhibit an opposite phenotype. Supplementing the media containing immobile Fe with fraxetin partially rescues the s8h mutants
Arabidopsis thaliana
metabolism
the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway. Fraxetin secretion is a decisive factor for calcicole-calcifuge behavior (i.e. the ability/inability to thrive on alkaline soils) of plants
Arabidopsis thaliana
physiological function
the enzyme participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway. Fraxetin secretion is a decisive factor for calcicole-calcifuge behavior (i.e. the ability/inability to thrive on alkaline soils) of plants
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
the enzyme is highly induced in roots of Fe-deficient plants both at the transcript and protein levels
up
Other publictions for EC 1.14.11.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748335
Siwinska
Scopoletin 8-hydroxylase a no ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
J. Exp. Bot.
69
1735-1748
2018
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1
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1
1
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1
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2
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7
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1
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1
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4
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1
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1
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1
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4
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1
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1
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1
1
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1
1
748735
Rajniak
Biosynthesis of redox-active ...
Arabidopsis thaliana
Nat. Chem. Biol.
14
442-450
2018
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1
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1
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1
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1
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1
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1
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1
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2
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-
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1
1
1
1
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-
748994
Tsai
Scopoletin 8-hydroxylase-medi ...
Arabidopsis thaliana
Plant Physiol.
177
194-207
2018
-
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1
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4
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1
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3
1
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