Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.62 extracted from

  • Vialart, G.; Hehn, A.; Olry, A.; Ito, K.; Krieger, C.; Larbat, R.; Paris, C.; Shimizu, B.; Sugimoto, Y.; Mizutani, M.; Bourgaud, F.
    A 2-oxoglutarate-dependent dioxygenase from Ruta graveolens L. Exhibits p-coumaroyl CoA 2-hydroxylase activity (C2H) A missing step in the synthesis of umbelliferone in plants (2012), Plant J., 70, 460-470 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Nicotiana benthamiana Ruta graveolens

Inhibitors

Inhibitors Comment Organism Structure
psoralen
-
Ruta graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
trans-feruloyl-CoA pH 7.2, 30°C Ruta graveolens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ruta graveolens no substrates: cinnamic acid, caffeic acid, p-coumaric acid, ferulic acid, cinnamoyl-CoA, caffeoyl-CoA ?
-
?
trans-4-coumaroyl-CoA + 2-oxoglutarate + O2 Ruta graveolens
-
2,4-dihydroxycinnamoyl-CoA + succinate + CO2
-
?
trans-feruloyl-CoA + 2-oxoglutarate + O2 Ruta graveolens reaction of EC 1.14.11.61 trans-6-hydroxyferuloyl-CoA + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Ruta graveolens H9LJF9 bifunctional enzyme, catalyzes the activities of EC 1.14.11.61 and EC 1.14.11.62 with equal activities
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf expression is induced by UV light Ruta graveolens
-
petiole expression is induced by UV light Ruta graveolens
-
root
-
Ruta graveolens
-
stem
-
Ruta graveolens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: cinnamic acid, caffeic acid, p-coumaric acid, ferulic acid, cinnamoyl-CoA, caffeoyl-CoA Ruta graveolens ?
-
?
trans-4-coumaroyl-CoA + 2-oxoglutarate + O2
-
Ruta graveolens 2,4-dihydroxycinnamoyl-CoA + succinate + CO2
-
?
trans-feruloyl-CoA + 2-oxoglutarate + O2 reaction of EC 1.14.11.61 Ruta graveolens trans-6-hydroxyferuloyl-CoA + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
CYP98A22
-
Ruta graveolens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.111
-
psoralen pH 7.2, 30°C Ruta graveolens

Expression

Organism Comment Expression
Ruta graveolens in leaf and petiole, expression is induced by UV light up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.014
-
trans-feruloyl-CoA pH 7.2, 30°C Ruta graveolens