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Literature summary for 1.14.11.66 extracted from

  • Verrier, L.; Escaffit, F.; Chailleux, C.; Trouche, D.; Vandromme, M.
    A new isoform of the histone demethylase JMJD2A/KDM4A is required for skeletal muscle differentiation (2011), PLoS Genet., 7, e1001390 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.66

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM4A, short isoform of JMJD2A, DELTAN-JMJD2A, expression analysis, expression profiling of C2C12 cells, overview. DELTAN1-JMJD2A and DELTAN2-JMJD2A mRNAs are expressed at about 20% of the amount of the full-length mRNAs Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information expression profiling of C2C12 cells following transfection with siRNAs against both isoforms of JMJD2A, transfected C2C12 cells with siRNAs that target exon 9 or exon 10 (sie9 and sie10) are maintained in proliferation medium for 24 h before shifting them to differentiation medium for 36 hours. The siRNAs do not affect JMJD2B and JMJD2C mRNA. Genes Actc1, Tnni1, Ttn, Myog, and Ckm, are considered as deregulated upon JMJD2A knockdown, as compared to a control siRNA Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
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 Homo sapiens 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens H3K9 demethylation by DELTAN-JMJD2A, e.g. on the Myog promoter, allows the removal of repressive chromatin marks, genome-wide analysis of JMJD2A targets, transcriptional profiling studies, overview ?
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 ?
[histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
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 [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2
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 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
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 [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O75164
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Source Tissue

Source Tissue Comment Organism Textmining
myoblast
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 Homo sapiens
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myotube
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 Homo sapiens
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skeletal muscle the short isoform of JMJD2A is upregulated during muscle differentiation, expression analysis and pattern, overview Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information H3K9 demethylation by DELTAN-JMJD2A, e.g. on the Myog promoter, allows the removal of repressive chromatin marks, genome-wide analysis of JMJD2A targets, transcriptional profiling studies, overview Homo sapiens ?
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 ?
additional information the bifunctional enzyme is active on H3K9me3/me2 and H3K36me3/me2 (EC 1.14.11.69) substrates Homo sapiens ?
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 ?
[histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2
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 Homo sapiens [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
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 ?

Synonyms

Synonyms Comment Organism
histone demethylase JMJD2A/KDM4A
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 Homo sapiens
JMJD2A
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 Homo sapiens
KDM4A
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 Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the JMJD2/KDM4 subfamily (JMJD2A, B, C and D) specifically demethylates H3K9 and H3K36 either di- or trimethylated Homo sapiens
malfunction siRNA silencing of DELTAN-JMJD2A results in drastic impairment of MHC expression and myotube formation, the Myog promoter is a specific target of DElTAN-JMJD2A. Genome-wide expression profiling and exon-specific siRNA knockdown indicate that, in contrast to the full-length protein, the N-terminal demethylase domain is necessary for myotube formation and muscle-specific gene expression Homo sapiens
physiological function a distinct short isoform, DELTAN-JMJD2A, of the histone demethylase JMJD2A/KDM4A is required for skeletal muscle differentiation, induction of the DELTAN-JMJD2A isoform at the onset of differentiation. In proliferating myoblasts, muscle specific genes are silenced by epigenetic modifications at their promoters, including histone H3K9 methylation. Derepression of the promoter of the gene encoding the myogenic factor myogenin (Myog) through demethylation of H3K9 residues is a key for initiation of muscle differentiation. By directing the removal of repressive chromatin marks at the Myog promoter, enzyme DELTAN-JMJD2A promotes transcriptional activation of the Myog gene and thus contributes to initiation of muscle-specific gene expression. DELTAN-JMJD2A is essential for differentiation and acts as an activator Homo sapiens