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Literature summary for 1.14.11.67 extracted from

  • Yu, X.; Chen, H.; Zuo, C.; Jin, X.; Yin, Y.; Wang, H.; Jin, M.; Ozato, K.; Xu, S.
    Chromatin remodeling demethylating H3K4me3 of type I IFNs gene by Rbp2 through interacting with Piasy for transcriptional attenuation (2017), FASEB J., 32, 552-567 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.67

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM5A, recombinant coexpression of wild-type and mutant FLAG-tagged Piasy and HA-tagged Rbp2 from pcDNA3.1(+) HA expression vector and p33 Flag CMV7.1 expression vector, respectively, in HEK-293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
H483G/E485Q site-directed mutagenesis Mus musculus
K152A site-directed mutagenesis Mus musculus
K152E site-directed mutagenesis Mus musculus
additional information RBP2 enzyme knockout by transfection of siRNA, that targets Rbp2, into Piasy+/+ MEFs Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatin
-
 Mus musculus 785
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for catalysis Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone H3 N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 Mus musculus
-
 histone H3 N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
additional information Mus musculus protein-protein interaction anaysis of wild-type and mutants and interactional domains between Rbp2 and Piasy, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q3UXZ9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast mouse embryonic fibroblasts, MEFs Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H3 N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2
-
 Mus musculus histone H3 N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
additional information protein-protein interaction anaysis of wild-type and mutants and interactional domains between Rbp2 and Piasy, overview Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
KDM5A
-
 Mus musculus
RBP2
-
 Mus musculus
retinoblastoma binding protein 2
-
 Mus musculus

General Information

General Information Comment Organism
malfunction overexpression of Rbp2, but not its enzymatically inactive mutant Rbp2H483G/E485Q, retards the transcription activities of IFNI, whereas small interfering RNA-mediated or short hairpin RNA-mediated knockdown of Rbp2 enhances IFNI promoter responses Mus musculus
physiological function retinoblastoma binding protein 2 (Rbp2) is a H3K4me3 demethylase that interacts with Piasy, a Pias (protein inhibitor of activated signal transducer and activator of transcription) family member, which possesses the ability to suppress IFNI transcriptions in mouse embryonic fibroblasts (MEFs). The H3K4me3 levels, one activation mark of genes, in MEFsthat are stimulated by poly(I:C), are impaired by Piasy in the IFN-beta gene. Piasy binds to the Jmjc domain (residues 451-503) of Rbp2 via its PINIT domain (residues 101-218), which is consistent with the domain required for their attenuation of transcription and H3K4me3 levels of IFNI genes. Piasy may prevent exaggerated transcription of IFNI by Rbp2-mediated demethylation of H3K4me3 of IFNI, avoiding excessive immune responses. Piasy reduces H3K4me3 levels of IFNI genes upon activation in MEFs, the Jmjc domain of Rbp2 and the PINIT domain of Piasy are prerequisites. Demethylase activity of Rbp2, but not DNA contact by K152, is indispensable Mus musculus