Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required | x Ascocenda |
Cloned (Comment) | Organism |
---|---|
gene F3H cloned from petals, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21 | x Ascocenda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.207 | - |
naringenin | pH 8.0, 45°C | x Ascocenda |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for catalytic activity | x Ascocenda |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | x Ascocenda | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | x Ascocenda NK-2012 | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | x Ascocenda | - |
dihydrokaempferol + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | x Ascocenda NK-2012 | - |
dihydrokaempferol + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
x Ascocenda | J9QYL0 | var. Subun | - |
x Ascocenda NK-2012 | J9QYL0 | var. Subun | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | enzyme expression analysis of AcF3H at different developmental stages and in different parts of the flower, overview. The highest enzyme expression occurs when the petals turned pale-orange | x Ascocenda | - |
petal | - |
x Ascocenda | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
x Ascocenda | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
x Ascocenda NK-2012 | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | - |
x Ascocenda | dihydrokaempferol + succinate + CO2 | - |
? | |
naringenin + 2-oxoglutarate + O2 | - |
x Ascocenda NK-2012 | dihydrokaempferol + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41220, sequence calculation, x * 41500, recombinant enzyme, SDS-PAGE | x Ascocenda |
Synonyms | Comment | Organism |
---|---|---|
AcF3H | - |
x Ascocenda |
F3H | - |
x Ascocenda |
flavanone 3-hydroxylase | - |
x Ascocenda |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant enzyme | x Ascocenda |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | recombinant enzyme, stable at 20-45°C, loss of activity above 45°C, inactivation at 60°C | x Ascocenda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | x Ascocenda |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
x Ascocenda | sequence calculation | - |
5.1 |