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Literature summary for 1.14.11.9 extracted from

  • Song, X.; Diao, J.; Ji, J.; Wang, G.; Guan, C.; Jin, C.; Wang, Y.
    Molecular cloning and identification of a flavanone 3-hydroxylase gene from Lycium chinense, and its overexpression enhances drought stress in tobacco (2016), Plant Physiol. Biochem., 98, 89-100 .
    View publication on PubMed
Search for more literature for 1.14.11.9

Cloned(Commentary)

Cloned (Comment) Organism
gene LcF3H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21(DE3), recombinant overexpression in leaves of Nicotiana tabacum cv. Xanthinc via transformation with Agrobacterium tumefaciens strain EHA105, overexpression of heterogenous LcF3H enhances the expression levels of downstream genes but does not cause alteration in the expression of upstream genes including endogenous F3H of Nicotiana tabacum. The enzyme's overexpression does cause a significant increase in the content of three major flavan-3-ols, catechin, epicatechin and epigallocatechin Lycium chinense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2 Lycium chinense
-
 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2 Lycium chinense
-
 dihydrokaempferol + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Lycium chinense A0A068EM79
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Lycium chinense
-
leaf primordial leaf, young leaf (highest level), and mature leaf Lycium chinense
-
additional information tissue pattern of enzyme expression, overview Lycium chinense
-
root low expression level Lycium chinense
-
seedling
-
 Lycium chinense
-
stem
-
 Lycium chinense
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 Lycium chinense a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Lycium chinense dihydrokaempferol + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
flavanone 3-hydroxylase
-
 Lycium chinense
LcF3H
-
 Lycium chinense

Expression

Organism Comment Expression
Lycium chinense the transcript levels of LcF3H shows significant decrease when plants are treated by exogenous nordihydroguaiaretic acid (100 mM) application together with drought stress treatment comparing to that treated with drought stress alone, the decrease in LcF3H expression under drought stress is less in overexpressing lines compared to the control plants down
Lycium chinense positive correlation between absisic acid and LcF3H expression level additional information

General Information

General Information Comment Organism
metabolism positive correlation between absisic acid and LcF3H expression level Lycium chinense
physiological function the flavanone 3-hydroxylase (F3H) gene which encodes flavanone 3-hydroxylase, is essential in flavonoids biosynthetic pathway. Lycium chinense (L. chinense) is a deciduous woody perennial halophyte that grows under a large variety of environmental conditions and survives under extreme drought stress, possible relationship between the oxidative damage and the regulation of LcF3H gene expression in Lycium chinense under drought stress Lycium chinense