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Literature summary for 1.14.11.B16 extracted from

  • Fraser, P.D.; Shimada, H.; Misawa, N.
    Enzymic confirmation of reactions involved in routes to astaxanthin formation, elucidated using a direct substrate in vitro assay (1998), Eur. J. Biochem., 252, 229-236.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Agrobacterium aurantiacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
beta-carotene 30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate Agrobacterium aurantiacum
0.002
-
echinenone 30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate Agrobacterium aurantiacum
0.0083
-
4-oxozeaxanthin 30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate Agrobacterium aurantiacum
0.01
-
zeaxanthin 30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate Agrobacterium aurantiacum
0.0143
-
3-hydroxyechinenone 30°C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate Agrobacterium aurantiacum

Organism

Organism UniProt Comment Textmining
Agrobacterium aurantiacum P54972
-
-
Agrobacterium aurantiacum N81106 P54972
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyechinenone + acceptor + O2
-
Agrobacterium aurantiacum phoenicoxanthin + reduced acceptor
-
?
3-hydroxyechinenone + acceptor + O2
-
Agrobacterium aurantiacum N81106 phoenicoxanthin + reduced acceptor
-
?
4-oxozeaxanthin + acceptor + O2
-
Agrobacterium aurantiacum astaxanthin + reduced acceptor
-
?
4-oxozeaxanthin + acceptor + O2
-
Agrobacterium aurantiacum N81106 astaxanthin + reduced acceptor
-
?
beta-carotene + acceptor + O2
-
Agrobacterium aurantiacum canthaxanthin + reduced acceptor
-
?
beta-carotene + acceptor + O2
-
Agrobacterium aurantiacum N81106 canthaxanthin + reduced acceptor
-
?
echinenone + acceptor + O2
-
Agrobacterium aurantiacum canthaxanthin + reduced acceptor
-
?
echinenone + acceptor + O2
-
Agrobacterium aurantiacum N81106 canthaxanthin + reduced acceptor
-
?
zeaxanthin + acceptor + O2
-
Agrobacterium aurantiacum adonixanthin + reduced acceptor
-
?
zeaxanthin + acceptor + O2
-
Agrobacterium aurantiacum N81106 adonixanthin + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
beta-carotene ketolase ambiguous Agrobacterium aurantiacum
CrtW
-
Agrobacterium aurantiacum