Literature summary for 1.14.12.11 extracted from

Zhang, N.; Stewart, B.G.; Moore, J.C.; Greasham, R.L.; Robinson, D.K.; Buckland, B.C.; Lee, C.
Directed evolution of toluene dioxygenase from Pseudomonas putida for improved selectivity toward cis-indandiol during indene bioconversion (2000), Metab. Eng., 2, 339-348.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	mutants SP6-1A, SP6-1G, and SP14-4H (created by error-prone PCR mutagenesis) show decreased 1-indenol production in comparison to the wild type enzyme	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
indene + NADH + H+ + O2	-	Pseudomonas putida	cis-(1S, 2R)-indandiol + 1-indenol + 1-indanone + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
TDO	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Pseudomonas putida

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Release 2023.1 (January 2023)