Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
F366 | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas putida |
I324F | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas putida |
T365N | the mutant shows slightly reduced activity compared to the wild type enzyme | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-dinitrobenzene + NADH + H+ + O2 | - |
Pseudomonas putida | ? | - |
? | |
bromobenzene + NADH + H+ + O2 | - |
Pseudomonas putida | cis-(1S,2S)-3-bromocyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
indene + NADH + H+ + O2 | - |
Pseudomonas putida | cis-(1S,2R)-indandiol + (1S)-indenol + NAD+ | - |
? | |
propylbenzene + NADH + H+ + O2 | - |
Pseudomonas putida | cis-(1S,2R)-3-propylcyclohexa-3,5-diene-1,2-diol + cis-(1S,2R)-3-[(1R)-1-hydroxypropyl]cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
styrene + NADH + H+ + O2 | - |
Pseudomonas putida | cis-2,3-dihydroxy-1-vinylcyclohexa-4,6-diene + (R)1-phenyl-1,2-ethanediol + NAD+ | - |
? | |
toluene + NADH + H+ + O2 | - |
Pseudomonas putida | (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TDO | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas putida |