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Literature summary for 1.14.12.12 extracted from

  • Ensley, B.D.; Haigler, B.E.
    Naphthalene dioxygenase from Pseudomonas NCIB 9816 (1990), Methods Enzymol., 188, 46-52.
    View publication on PubMed
Search for more literature for 1.14.12.12

General Stability

General Stability Organism
10% ethanol and 10% glycerol are required for stability Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas sp. 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
naphthalene + NADH + O2 Pseudomonas sp.
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
component B i.e. the terminal oxygenase ISPNAP, DEAE-Sephadex, DEAE-cellulose, octyl-Sepharose, presence of 10% ethanol and 10% glycerol is required to maintain stability Pseudomonas sp.
ferredoxinNAP reductase, Blue Sepharose, DEAE-cellulose Pseudomonas sp.
purification of ferredoxinNAP reductase, ferredoxinNAP and ISPNAP Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
naphthalene + NADH + O2
-
 Pseudomonas sp. cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
naphthalene + NADH + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas sp.
additional information spinach ferredoxin cannot replace ferredoxinNAP Pseudomonas sp.
NADH
-
 Pseudomonas sp.