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Literature summary for 1.14.12.12 extracted from

  • Ferraro, D.J.; Okerlund, A.; Brown, E.; Ramaswamy, S.
    One enzyme, many reactions structural basis for the various reactions catalyzed by naphthalene 1,2-dioxygenase (2017), IUCrJ, 4, 648-656 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DE3 Star cells Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1.9-2.2 M ammonium sulfate, 4-6% (w/v) dioxane, 100 mM MES, pH 5.0-5.8 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
naphthalene + NADH + H+ + O2 Pseudomonas sp.
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
naphthalene + NADH + H+ + O2 Pseudomonas sp. C18
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. P0A111
-
-
Pseudomonas sp. C18 P0A111
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, octyl Sepharose column chromatography, and hydroxyapatite ion-exchange column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloronaphthalene + NADH + H+ + O2
-
 Pseudomonas sp. (1R,2S)-5-chloro-1,2-dihydronaphthalene-1,2-diol + (1S,2R)-8-chloro-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1-chloronaphthalene + NADH + H+ + O2
-
 Pseudomonas sp. C18 (1R,2S)-5-chloro-1,2-dihydronaphthalene-1,2-diol + (1S,2R)-8-chloro-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
benzamide + NADH + H+ + O2
-
 Pseudomonas sp. ?
-
 ?
benzamide + NADH + H+ + O2
-
 Pseudomonas sp. C18 ?
-
 ?
ethylbenzene + NADH + H+ + O2
-
 Pseudomonas sp. (S)-1-phenylethanol + (R)-1-phenylethanol + NAD+
-
 ?
ethylbenzene + NADH + H+ + O2
-
 Pseudomonas sp. C18 (S)-1-phenylethanol + (R)-1-phenylethanol + NAD+
-
 ?
ethylphenyl sulfide + NADH + H+ + O2
-
 Pseudomonas sp. (R)-ethylphenyl sulfoxide + NAD+
-
 ?
ethylphenyl sulfide + NADH + H+ + O2
-
 Pseudomonas sp. C18 (R)-ethylphenyl sulfoxide + NAD+
-
 ?
indan + NADH + H+ + O2
-
 Pseudomonas sp. (S)-1-indanol + (R)-1-indanol + NAD+
-
 ?
indene + NADH + H+ + O2
-
 Pseudomonas sp. (S)-1-indenol + (R)-1-indenol + NAD+
-
 ?
indole-3-acetate + NADH + H+ + O2
-
 Pseudomonas sp. ?
-
 ?
naphthalene + NADH + H+ + O2
-
 Pseudomonas sp. (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
naphthalene + NADH + H+ + O2
-
 Pseudomonas sp. C18 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
phenetole + NADH + H+ + O2
-
 Pseudomonas sp. phenol + vinyloxybenzene + (S)-ethylphenyl sulfoxide + NAD+
-
 ?
styrene + NADH + H+ + O2
-
 Pseudomonas sp. (R)-1-phenylethane-1,2-diol + (S)-1-phenylethane-1,2-diol + NAD+
-
 ?
thioanisole + NADH + H+ + O2
-
 Pseudomonas sp. methyl phenyl sulfoxide + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
NDO
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas sp.
[2Fe-2S]-center
-
 Pseudomonas sp.