BRENDA - Enzyme Database
show all sequences of 1.14.12.13

Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS

Haak, B.; Fetzner, S.; Lingens, F.; J. Bacteriol. 177, 667-675 (1995)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
Escherichia coli TG1, JM105, MV1190 and Pseudomonas putida KT2440 are used as host strains
Burkholderia cepacia
Engineering
Protein Variants
Commentary
Organism
additional information
several mutants of Pseudomonas cepacia, putida and sp. are used
Burkholderia cepacia
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
iron-sulfur protein
Burkholderia cepacia
Sulfide
iron-sulfur protein
Burkholderia cepacia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19500
-
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE
Burkholderia cepacia
37100
-
1 * 37100, NADH:acceptor reductase component, SDS-PAGE
Burkholderia cepacia
52400
-
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE
Burkholderia cepacia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-chlorobenzoate + NADH + O2
Burkholderia cepacia
initial step in the degradation of aromatic compounds
catechol + chloride + NAD+ + CO2
-
Burkholderia cepacia
?
Organism
Organism
UniProt
Commentary
Textmining
Burkholderia cepacia
-
2CBS, isolated from water
-
Purification (Commentary)
Purification (Commentary)
Organism
anion-exchange
Burkholderia cepacia
Reaction
Reaction
Commentary
Organism
Reaction ID
a 2-halobenzoate + NADH + H+ + O2 = catechol + a halide anion + NAD+ + CO2
two-component nonheme iron dioxygenase system of oxygenase system class IB dioxygenase: NADH:acceptor reductase containing both FAD and cloroplast-type 2Fe-2S and Rieske-type 2Fe-2S-containing oxygenase component
Burkholderia cepacia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-bromobenzoate + NADH + O2
-
439075
Burkholderia cepacia
catechol + bromide + NAD+ + CO2
-
439075
Burkholderia cepacia
?
2-chlorobenzoate + NADH + O2
-
439075
Burkholderia cepacia
catechol + chloride + NAD+ + CO2
-
439075
Burkholderia cepacia
?
2-chlorobenzoate + NADH + O2
initial step in the degradation of aromatic compounds
439075
Burkholderia cepacia
catechol + chloride + NAD+ + CO2
-
439075
Burkholderia cepacia
?
2-fluorobenzoate + NADH + O2
-
439075
Burkholderia cepacia
catechol + fluoride + NAD+ + CO2
-
439075
Burkholderia cepacia
?
2-iodobenzoate + NADH + O2
-
439075
Burkholderia cepacia
catechol + iodide + NAD+ + CO2
-
439075
Burkholderia cepacia
?
additional information
very broad substrate specificity
439075
Burkholderia cepacia
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 37100, NADH:acceptor reductase component, SDS-PAGE
Burkholderia cepacia
oligomer
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE
Burkholderia cepacia
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Burkholderia cepacia
NADH
-
Burkholderia cepacia
Cloned(Commentary) (protein specific)
Commentary
Organism
Escherichia coli TG1, JM105, MV1190 and Pseudomonas putida KT2440 are used as host strains
Burkholderia cepacia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Burkholderia cepacia
NADH
-
Burkholderia cepacia
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
several mutants of Pseudomonas cepacia, putida and sp. are used
Burkholderia cepacia
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
iron-sulfur protein
Burkholderia cepacia
Sulfide
iron-sulfur protein
Burkholderia cepacia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19500
-
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE
Burkholderia cepacia
37100
-
1 * 37100, NADH:acceptor reductase component, SDS-PAGE
Burkholderia cepacia
52400
-
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE
Burkholderia cepacia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-chlorobenzoate + NADH + O2
Burkholderia cepacia
initial step in the degradation of aromatic compounds
catechol + chloride + NAD+ + CO2
-
Burkholderia cepacia
?
Purification (Commentary) (protein specific)
Commentary
Organism
anion-exchange
Burkholderia cepacia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-bromobenzoate + NADH + O2
-
439075
Burkholderia cepacia
catechol + bromide + NAD+ + CO2
-
439075
Burkholderia cepacia
?
2-chlorobenzoate + NADH + O2
-
439075
Burkholderia cepacia
catechol + chloride + NAD+ + CO2
-
439075
Burkholderia cepacia
?
2-chlorobenzoate + NADH + O2
initial step in the degradation of aromatic compounds
439075
Burkholderia cepacia
catechol + chloride + NAD+ + CO2
-
439075
Burkholderia cepacia
?
2-fluorobenzoate + NADH + O2
-
439075
Burkholderia cepacia
catechol + fluoride + NAD+ + CO2
-
439075
Burkholderia cepacia
?
2-iodobenzoate + NADH + O2
-
439075
Burkholderia cepacia
catechol + iodide + NAD+ + CO2
-
439075
Burkholderia cepacia
?
additional information
very broad substrate specificity
439075
Burkholderia cepacia
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 37100, NADH:acceptor reductase component, SDS-PAGE
Burkholderia cepacia
oligomer
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE
Burkholderia cepacia
Other publictions for EC 1.14.12.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742665
Xu
-
Degradation of three monochlo ...
Pseudomonas sp. 2-CBA
Int. Biodeterior. Biodegrad.
120
192-202
2017
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-
1
-
-
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-
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1
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1
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1
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3
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1
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1
1
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1
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1
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-
704034
Baggi
-
Co-metabolism of di- and trich ...
Cupriavidus necator, Stenotrophomonas maltophilia
Int. Biodeter. Biodegrad.
62
57-64
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
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4
-
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2
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-
-
-
-
-
-
-
-
-
2
2
-
-
-
688859
Wittich
Growth of the genetically engi ...
Burkholderia cepacia, Burkholderia cepacia 2CBS
Microbiology
153
186-195
2007
-
-
1
-
-
-
-
-
-
-
-
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-
7
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2
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1
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-
-
-
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-
-
-
-
-
-
-
-
-
439078
Suzuki
Expression of 2-halobenzoate d ...
Burkholderia sp.
Gene
262
137-145
2001
-
-
1
-
1
-
-
-
-
-
-
1
-
10
-
-
-
-
-
-
6
-
6
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439079
Francisco
The chlorobenzoate dioxygenase ...
Burkholderia sp., Burkholderia sp. NK8
Microbiology
147
121-133
2001
-
-
1
-
1
-
-
-
-
2
-
2
-
21
-
-
-
1
-
-
3
-
12
-
-
1
-
-
-
-
1
-
2
-
-
-
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-
1
2
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
3
-
12
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
439077
Fava
2-Chlorobenzoic acid and 2,5-d ...
Pseudomonas sp., Pseudomonas sp. CPE2
Lett. Appl. Microbiol.
22
275-279
1996
-
-
-
-
-
-
2
2
-
1
-
4
-
2
-
-
-
-
-
-
2
-
8
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
2
-
1
-
4
-
-
-
-
-
-
2
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
439075
Haak
Cloning, nucleotide sequence, ...
Burkholderia cepacia
J. Bacteriol.
177
667-675
1995
-
-
1
-
1
-
-
-
-
2
3
1
-
18
-
-
1
1
-
-
-
-
6
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
2
3
1
-
-
-
1
-
-
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439076
Riedel
EPR, electron spin echo envelo ...
Burkholderia cepacia
J. Biol. Chem.
270
30869-30873
1995
-
-
-
-
-
-
-
-
4
2
-
1
-
15
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
2
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661983
Romanov
Pseudomonas aeruginosa 142 use ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa 142
J. Bacteriol.
176
3368-3374
1994
-
-
-
-
-
-
1
-
-
1
1
-
-
5
-
-
-
-
-
-
1
-
20
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
20
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439074
Fetzner
Purification and some properti ...
Burkholderia cepacia
J. Bacteriol.
174
279-290
1992
-
-
-
-
-
-
11
2
-
3
5
1
-
8
-
-
1
2
-
-
3
5
12
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
11
-
2
-
3
5
1
-
-
-
1
-
-
3
5
12
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439073
Fetzner
Degradation of 2-chlorobenzoat ...
Burkholderia cepacia
Biol. Chem. Hoppe-Seyler
370
1173 - 1182
1989
-
-
-
-
1
1
-
-
-
1
-
1
-
4
-
-
1
1
-
-
2
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-