BRENDA - Enzyme Database
show all sequences of 1.14.12.13

The chlorobenzoate dioxygenase genes of Burkholderia sp. strain NK8 involved in the catabolism of chlorobenzoates

Francisco, P.B.; Ogawa, N.; Suzuki, K.; Miyashita, K.; Microbiology 147, 121-133 (2001)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
several gene constructs are expressed in Escherichia coli DH5alpha, S17-1, S17-lambdapir and HMS174(DE3)
Burkholderia sp.
Engineering
Protein Variants
Commentary
Organism
additional information
disruptants of Burkholderia sp. NK8 are generated by omega cassette interposon mutagenesis: NDBA1, NCAD, NCRD and NBALZ. Mutagenesis shows the involvement of the cbeABCD genes in the oxidation of 2-, 3-, 4-chlorobenzoate and benzoate in NK8
Burkholderia sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
-
Burkholderia sp.
Sulfide
-
Burkholderia sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-chlorobenzoate + NADH + O2
Burkholderia sp.
-
catechol + chloride + NAD+ + CO2
-
Burkholderia sp.
?
2-chlorobenzoate + NADH + O2
Burkholderia sp. NK8
-
catechol + chloride + NAD+ + CO2
-
Burkholderia sp. NK8
?
Organism
Organism
UniProt
Commentary
Textmining
Burkholderia sp.
-
isolated from soil
-
Burkholderia sp. NK8
-
isolated from soil
-
Reaction
Reaction
Commentary
Organism
Reaction ID
a 2-halobenzoate + NADH + H+ + O2 = catechol + a halide anion + NAD+ + CO2
broad substrate specificity, ability to transform all 3 monochlorobenzoates almost equally well. Enzyme appears to lack absolute regioselectivity
Burkholderia sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0013
-
cells are grown on 3-chlorobenzoate, benzoate as substrate for assay
Burkholderia sp.
0.0024
-
recombinant cells are grown on LB/IPTG, benzoate as substrate for assay
Burkholderia sp.
0.0032
-
cells are grown on benzoate, benzoate as substrate for assay
Burkholderia sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
catechol + chloride + NAD+ + CO2
-
439079
Burkholderia sp.
?
2-chlorobenzoate + NADH + O2
no growth on 2-chlorobenzoate
439079
Burkholderia sp.
catechol + chloride + NAD+ + CO2
-
439079
Burkholderia sp.
?
2-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp. NK8
catechol + chloride + NAD+ + CO2
-
439079
Burkholderia sp. NK8
?
2-chlorobenzoate + NADH + O2
no growth on 2-chlorobenzoate
439079
Burkholderia sp. NK8
catechol + chloride + NAD+ + CO2
-
439079
Burkholderia sp. NK8
?
2-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
3-chlorocatechol + NAD+ + O2
-
439079
Burkholderia sp.
?
2-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp. NK8
3-chlorocatechol + NAD+ + O2
-
439079
Burkholderia sp. NK8
?
3-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
4-chlorocatechol + NAD+ + CO2
-
439079
Burkholderia sp.
?
3-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp. NK8
4-chlorocatechol + NAD+ + CO2
-
439079
Burkholderia sp. NK8
?
3-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
3-chlorocatechol + NAD+ + CO2
-
439079
Burkholderia sp.
?
4-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
4-chlorocatechol + NAD+ + CO2
-
439079
Burkholderia sp.
?
benzoate + NADH + O2
2-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid as unstable intermediate
439079
Burkholderia sp.
catechol + NAD+ + CO2
-
439079
Burkholderia sp.
?
benzoate + NADH + O2
2-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid as unstable intermediate
439079
Burkholderia sp. NK8
catechol + NAD+ + CO2
-
439079
Burkholderia sp. NK8
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Burkholderia sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
7.5
assay at
Burkholderia sp.
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Burkholderia sp.
NADH
-
Burkholderia sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
several gene constructs are expressed in Escherichia coli DH5alpha, S17-1, S17-lambdapir and HMS174(DE3)
Burkholderia sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Burkholderia sp.
NADH
-
Burkholderia sp.
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
disruptants of Burkholderia sp. NK8 are generated by omega cassette interposon mutagenesis: NDBA1, NCAD, NCRD and NBALZ. Mutagenesis shows the involvement of the cbeABCD genes in the oxidation of 2-, 3-, 4-chlorobenzoate and benzoate in NK8
Burkholderia sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
-
Burkholderia sp.
Sulfide
-
Burkholderia sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-chlorobenzoate + NADH + O2
Burkholderia sp.
-
catechol + chloride + NAD+ + CO2
-
Burkholderia sp.
?
2-chlorobenzoate + NADH + O2
Burkholderia sp. NK8
-
catechol + chloride + NAD+ + CO2
-
Burkholderia sp. NK8
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0013
-
cells are grown on 3-chlorobenzoate, benzoate as substrate for assay
Burkholderia sp.
0.0024
-
recombinant cells are grown on LB/IPTG, benzoate as substrate for assay
Burkholderia sp.
0.0032
-
cells are grown on benzoate, benzoate as substrate for assay
Burkholderia sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
catechol + chloride + NAD+ + CO2
-
439079
Burkholderia sp.
?
2-chlorobenzoate + NADH + O2
no growth on 2-chlorobenzoate
439079
Burkholderia sp.
catechol + chloride + NAD+ + CO2
-
439079
Burkholderia sp.
?
2-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp. NK8
catechol + chloride + NAD+ + CO2
-
439079
Burkholderia sp. NK8
?
2-chlorobenzoate + NADH + O2
no growth on 2-chlorobenzoate
439079
Burkholderia sp. NK8
catechol + chloride + NAD+ + CO2
-
439079
Burkholderia sp. NK8
?
2-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
3-chlorocatechol + NAD+ + O2
-
439079
Burkholderia sp.
?
2-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp. NK8
3-chlorocatechol + NAD+ + O2
-
439079
Burkholderia sp. NK8
?
3-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
4-chlorocatechol + NAD+ + CO2
-
439079
Burkholderia sp.
?
3-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp. NK8
4-chlorocatechol + NAD+ + CO2
-
439079
Burkholderia sp. NK8
?
3-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
3-chlorocatechol + NAD+ + CO2
-
439079
Burkholderia sp.
?
4-chlorobenzoate + NADH + O2
-
439079
Burkholderia sp.
4-chlorocatechol + NAD+ + CO2
-
439079
Burkholderia sp.
?
benzoate + NADH + O2
2-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid as unstable intermediate
439079
Burkholderia sp.
catechol + NAD+ + CO2
-
439079
Burkholderia sp.
?
benzoate + NADH + O2
2-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid as unstable intermediate
439079
Burkholderia sp. NK8
catechol + NAD+ + CO2
-
439079
Burkholderia sp. NK8
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Burkholderia sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
7.5
assay at
Burkholderia sp.
Other publictions for EC 1.14.12.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742665
Xu
-
Degradation of three monochlo ...
Pseudomonas sp. 2-CBA
Int. Biodeterior. Biodegrad.
120
192-202
2017
-
-
1
-
-
-
-
-
-
-
-
1
-
1
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-
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1
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3
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1
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1
1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
704034
Baggi
-
Co-metabolism of di- and trich ...
Cupriavidus necator, Stenotrophomonas maltophilia
Int. Biodeter. Biodegrad.
62
57-64
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
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4
-
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2
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-
-
-
-
-
-
-
-
-
2
2
-
-
-
688859
Wittich
Growth of the genetically engi ...
Burkholderia cepacia, Burkholderia cepacia 2CBS
Microbiology
153
186-195
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
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-
-
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-
2
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439078
Suzuki
Expression of 2-halobenzoate d ...
Burkholderia sp.
Gene
262
137-145
2001
-
-
1
-
1
-
-
-
-
-
-
1
-
10
-
-
-
-
-
-
6
-
6
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439079
Francisco
The chlorobenzoate dioxygenase ...
Burkholderia sp., Burkholderia sp. NK8
Microbiology
147
121-133
2001
-
-
1
-
1
-
-
-
-
2
-
2
-
21
-
-
-
1
-
-
3
-
12
-
-
1
-
-
-
-
1
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
3
-
12
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
439077
Fava
2-Chlorobenzoic acid and 2,5-d ...
Pseudomonas sp., Pseudomonas sp. CPE2
Lett. Appl. Microbiol.
22
275-279
1996
-
-
-
-
-
-
2
2
-
1
-
4
-
2
-
-
-
-
-
-
2
-
8
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
2
-
1
-
4
-
-
-
-
-
-
2
-
8
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
439075
Haak
Cloning, nucleotide sequence, ...
Burkholderia cepacia
J. Bacteriol.
177
667-675
1995
-
-
1
-
1
-
-
-
-
2
3
1
-
18
-
-
1
1
-
-
-
-
6
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
2
3
1
-
-
-
1
-
-
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439076
Riedel
EPR, electron spin echo envelo ...
Burkholderia cepacia
J. Biol. Chem.
270
30869-30873
1995
-
-
-
-
-
-
-
-
4
2
-
1
-
15
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
2
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661983
Romanov
Pseudomonas aeruginosa 142 use ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa 142
J. Bacteriol.
176
3368-3374
1994
-
-
-
-
-
-
1
-
-
1
1
-
-
5
-
-
-
-
-
-
1
-
20
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
1
-
20
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439074
Fetzner
Purification and some properti ...
Burkholderia cepacia
J. Bacteriol.
174
279-290
1992
-
-
-
-
-
-
11
2
-
3
5
1
-
8
-
-
1
2
-
-
3
5
12
2
-
1
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
11
-
2
-
3
5
1
-
-
-
1
-
-
3
5
12
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439073
Fetzner
Degradation of 2-chlorobenzoat ...
Burkholderia cepacia
Biol. Chem. Hoppe-Seyler
370
1173 - 1182
1989
-
-
-
-
1
1
-
-
-
1
-
1
-
4
-
-
1
1
-
-
2
-
2
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-