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Literature summary for 1.14.12.17 extracted from
- Nitric-oxide dioxygenase function of human cytoglobin with cellular reductants and in rat hepatocytes (2010), J. Biol. Chem., 285, 23850-23857.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | stimulates NOD activity, reduces the oxidized Cygb-NOD intermediate with a second order rate of 1000 M/s, Km is 0.25 mM | Homo sapiens |  |
| cytochrome b5 | stimulates NOD activity, reduces the oxidized Cygb-NOD intermediate with a second order rate of 3 x 1000000 M/s, Km is 0.3 mM | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of human cytoglobin in rat hepatocytes and effects on the cell NO metabolism, overview. Expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CO | a competitive inhibitor | Homo sapiens | |
| cyanide | causes slow inactivation | Homo sapiens | |
| additional information | anions competitively inhibit activity. Increasing the sodium phosphate concentration or including sodium phosphate or NaCl in a 25 mM sodium citrate buffer also competitively inhibits the activity and increases the Km value of ascorbate | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten behavior of the Cygb-NOD activity with ascorbate | Homo sapiens | |
| 0.00002 | - |
O2 | pH 7.0, 37°C | Homo sapiens | |
| 0.00004 | - |
nitric oxide | pH 7.0, 37°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatocyte | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + 2 O2 + NADPH | via an Cygb-NOD intermediate, putative ascorbate-binding site in Cygb, overview | Homo sapiens | 2 nitrate + NADP+ + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cygb | - |
Homo sapiens |
| cytoglobin | - |
Homo sapiens |
| nitric-oxide dioxygenase | - |
Homo sapiens |
| NO dioxygenase | - |
Homo sapiens |
| NOD | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme is distinct from the microsomal NOD activity | Homo sapiens |
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