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Literature summary for 1.14.12.18 extracted from

  • Hurtubise, Y.; Barriault, D.; Sylvestre, M.
    Involvement of the terminal oxygenase beta subunit in the biphenyl dioxygenase reactivity pattern toward chlorobiphenyls (1998), J. Bacteriol., 180, 5828-5835.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli, strain M15 Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
T375N conversion of sequence to corresponding sequence of Pseudomonas sp. strain LB400 Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. LB400
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-dichlorobiphenyl + NADH + O2
-
Pseudomonas sp. 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
?
2,2'-dichlorobiphenyl + NADH + O2
-
Comamonas testosteroni 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
?
2,2'-dichlorobiphenyl + NADH + O2
-
Pseudomonas sp. LB400 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 2,3-dihydroxy-2'-chlorobiphenyl + NAD+ + HCl
-
?
2,5,2',5'-tetrachlorobiphenyl + NADH + O2
-
Pseudomonas sp. cis-3,4-dihydroxy-2,5-dichloro-1-[2',5'-dichlorophenyl]-cyclohexa-1,5-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
?
2,5,2',5'-tetrachlorobiphenyl + NADH + O2
-
Pseudomonas sp. LB400 cis-3,4-dihydroxy-2,5-dichloro-1-[2',5'-dichlorophenyl]-cyclohexa-1,5-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
?
2,5-dichlorobiphenyl + NADH + O2
-
Pseudomonas sp. cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
?
2,5-dichlorobiphenyl + NADH + O2 no 3,4-dihydrodiol as product Comamonas testosteroni cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
?
2,5-dichlorobiphenyl + NADH + O2
-
Pseudomonas sp. LB400 cis-2',3'-dihydroxy-1'-(2,5-dichlorophenyl)-cyclohexa-4',6'-diene + 3,4-dihydroxy-1-phenylcyclohexa-1,5-diene + NAD+ + HCl
-
?
3,3'-dichlorobiphenyl + NADH + O2
-
Pseudomonas sp. 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
?
3,3'-dichlorobiphenyl + NADH + O2
-
Comamonas testosteroni 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
?
3,3'-dichlorobiphenyl + NADH + O2
-
Pseudomonas sp. LB400 5,6-dihydroxy-1-phenylcyclohexa-1,3-diene + 4,5-dihydroxy-1-phenylcyclohexa-1,2-diene + NAD+ + HCl
-
?
biphenyl + NAD(P)H + O2
-
Comamonas testosteroni 2,3-dihydro-dihydroxybiphenyl + NAD(P)+
-
?