Any feedback?
Literature summary for 1.14.12.22 extracted from
- High-level expression, purification and characterization of carbazole dioxygenase, a three components dioxygenase, of Pseudomonas GBS.5 (2015), Biotechnol. Lett., 37, 1945-1952 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | indispensable for catalytic activity | Pseudomonas sp. GBS.5 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. GBS.5 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9H-carbazole + NADH + H+ + O2 | Pseudomonas sp. GBS.5 | - |
2'-aminobiphenyl-2,3-diol + NAD+ | - |
? |  |
| 9H-carbazole + NADPH + H+ + O2 | Pseudomonas sp. GBS.5 | - |
2'-aminobiphenyl-2,3-diol + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. GBS.5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione bead chromatography | Pseudomonas sp. GBS.5 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9H-carbazole + NADH + H+ + O2 | - |
Pseudomonas sp. GBS.5 | 2'-aminobiphenyl-2,3-diol + NAD+ | - |
? | |
| 9H-carbazole + NADPH + H+ + O2 | - |
Pseudomonas sp. GBS.5 | 2'-aminobiphenyl-2,3-diol + NADP+ | - |
? | |
| anthracene + NADH + H+ + O2 | - |
Pseudomonas sp. GBS.5 | ? | - |
? | |
| dibenzothiophene + NADH + H+ + O2 | - |
Pseudomonas sp. GBS.5 | ? | - |
? | |
| fluoranthene + NADH + H+ + O2 | - |
Pseudomonas sp. GBS.5 | ? | - |
? | |
| additional information | the enzyme does not degrade alkanes like pentadecane, hexadecane, heptadecane, octadecane, nonadecane and docosane | Pseudomonas sp. GBS.5 | ? | - |
? | |
| phenanthrene + NADH + H+ + O2 | - |
Pseudomonas sp. GBS.5 | ? | - |
? | |
| pyrene + NADH + H+ + O2 | - |
Pseudomonas sp. GBS.5 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CarAa | - |
Pseudomonas sp. GBS.5 |
| carbazole dioxygenase | - |
Pseudomonas sp. GBS.5 |
| CARDO | - |
Pseudomonas sp. GBS.5 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Pseudomonas sp. GBS.5 | |
| NADPH | - |
Pseudomonas sp. GBS.5 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
