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Literature summary for 1.14.12.24 extracted from

  • Leungsakul, T.; Keenan, B.G.; Yin, H.; Smets, B.F.; Wood, T.K.
    Saturation mutagenesis of 2,4-DNT dioxygenase of Burkholderia sp. strain DNT for enhanced dinitrotoluene degradation (2005), Biotechnol. Bioeng., 92, 416-426.
    View publication on PubMed
Search for more literature for 1.14.12.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TG1 cells Burkholderia sp.

Protein Variants

Protein Variants Comment Organism
I204L the mutant transforms both 2,6-dinitrotoluene and 2,4-dinitrotoluene 2fold faster than the wild type enzyme and exhibits activity with 2,5- and 2,3-dinitrotoluene Burkholderia sp.
I204Y the mutant transforms both 2,6-dinitrotoluene 2,5fold faster than the wild type enzyme and exhibits activity with 2,5- and 2,3-dinitrotoluene Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dinitrotoluene + NADH + O2 Burkholderia sp.
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
2,4-dinitrotoluene + NADH + O2 Burkholderia sp. DNT
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
additional information Burkholderia sp. no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene ?
-
 ?
additional information Burkholderia sp. DNT no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene ?
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia sp. Q6QUY3
-
-
Burkholderia sp. DNT Q6QUY3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrotoluene + NADH + O2
-
 Burkholderia sp. 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
2,4-dinitrotoluene + NADH + O2 best substrate Burkholderia sp. 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
2,4-dinitrotoluene + NADH + O2
-
 Burkholderia sp. DNT 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
2,4-dinitrotoluene + NADH + O2 best substrate Burkholderia sp. DNT 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
2,6-dinitrotoluene + NADH + O2
-
 Burkholderia sp. 3-methyl-4-nitrocatechol + nitrite + NAD+
-
 ?
2,6-dinitrotoluene + NADH + O2
-
 Burkholderia sp. DNT 3-methyl-4-nitrocatechol + nitrite + NAD+
-
 ?
2-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 2-nitrobenzyl alcohol + NAD+ + H2O
-
 ?
2-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. DNT 2-nitrobenzyl alcohol + NAD+ + H2O
-
 ?
4-nitrotoluene + NADH + O2
-
 Burkholderia sp. 4-methylcatechol + nitrite + NAD+
-
 ?
additional information no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene Burkholderia sp. ?
-
 ?
additional information no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene Burkholderia sp. DNT ?
-
 ?

Synonyms

Synonyms Comment Organism
2,4-DNT dioxygenase
-
 Burkholderia sp.
DDO
-
 Burkholderia sp.