Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109(DE3) cells | Burkholderia sp. |
expression in Escherichia coli | Burkholderia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADH + O2 | Burkholderia sp. | - |
4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
2,4-dinitrotoluene + NADH + O2 | Burkholderia sp. DNT | - |
4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
- |
- |
Burkholderia sp. | Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 | Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd | - |
Burkholderia sp. DNT | - |
- |
- |
Burkholderia sp. DNT | Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 | Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADH + O2 | - |
Burkholderia sp. | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
2,4-dinitrotoluene + NADH + O2 | - |
Burkholderia sp. DNT | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
2-nitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. | 2-nitrobenzyl alcohol + NAD+ + ? | - |
? | |
2-nitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 2-nitrobenzyl alcohol + NAD+ + ? | - |
? | |
3-nitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. | 3-nitrobenzyl alcohol + NAD+ + ? | - |
? | |
3-nitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 3-nitrobenzyl alcohol + NAD+ + ? | - |
? | |
4-nitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. | 4-nitrobenzyl alcohol + NAD+ + ? | - |
? | |
4-nitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 4-nitrobenzyl alcohol + NAD+ + ? | - |
? | |
acenaphthene + NADH + H+ + O2 | - |
Burkholderia sp. | 1-acenaphthenol + NAD+ + ? | - |
? | |
indan + NADH + H+ + O2 | - |
Burkholderia sp. | 1-indanol + 1-indanone + indene + NAD+ | - |
? | |
indan + NADH + H+ + O2 | - |
Burkholderia sp. | 1-indanol + ? + NAD+ | no formation of 1,2-dihydroxyindan | ? | |
indan + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 1-indanol + ? + NAD+ | no formation of 1,2-dihydroxyindan | ? | |
indene + NADH + H+ + O2 | - |
Burkholderia sp. | 1-indenol + 1,2-dihydroxyindan + NAD+ | - |
? | |
indole + NADH + H+ + O2 | - |
Burkholderia sp. | indigo + NAD+ + ? | - |
? | |
indoline + NADH + H+ + O2 | - |
Burkholderia sp. | indigo + ? + NAD+ | - |
? | |
indoline + NADH + H+ + O2 | - |
Burkholderia sp. DNT | indigo + ? + NAD+ | - |
? | |
indoline + NADH + H+ + O2 | - |
Burkholderia sp. | indole + NAD+ + ? | - |
? | |
additional information | no activity with anisole, carbazole, and nitrobenzene | Burkholderia sp. | ? | - |
? | |
additional information | no activity with anisole, carbazole, and nitrobenzene | Burkholderia sp. DNT | ? | - |
? | |
naphthalene + NADH + H+ + O2 | - |
Burkholderia sp. | (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ | - |
? | |
naphthalene + O2 | - |
Burkholderia sp. | cis-1,2-dihydroxy-1,2-dihydronaphthalene | product is formed in 92% enantiomeric excess | ? | |
naphthalene + O2 | - |
Burkholderia sp. DNT | cis-1,2-dihydroxy-1,2-dihydronaphthalene | product is formed in 92% enantiomeric excess | ? | |
phenetole + NADH + H+ + O2 | - |
Burkholderia sp. | phenol + NAD+ + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNT dioxygenase | - |
Burkholderia sp. |
dntA | - |
Burkholderia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Burkholderia sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | an Escherichia coli clone that expresses DNT dioxygenase converts dinitrotoluene to 4-methyl-5-nitrocatechol and naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene. The enzyme system exhibits similar substrate specificities as naphthalene dioxygenase, both can oxidize such compounds as indole, indan, indene, phenetole, and acenaphthene | Burkholderia sp. |