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Literature summary for 1.14.12.3 extracted from

  • Wackett, L.P.
    Benzene dioxygenase from Pseudomonas putida, ML2 (NCIB 12190) (1990), Methods Enzymol., 188, 52-60.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00782
-
NADH
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the terminal dioxygenase component contains two Fe2+ ions per molecule in addition to two [2Fe-2S] iron-sulfur clusters Pseudomonas putida
Iron the terminal dioxygenase component contains two Fe2+ ions per molecule in addition to two [2Fe-2S] iron-sulfur clusters. The ferredoxin component contains one [2Fe-2S] cluster Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da Pseudomonas putida
42000
-
alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da Pseudomonas putida
54500
-
alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzene + NADH + O2 Pseudomonas putida reaction in benzene catabolism ?
-
?
benzene + NADH + O2 Pseudomonas putida ML2 reaction in benzene catabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ML2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, stable for 6 months to 1 year Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene + NADH + O2
-
Pseudomonas putida cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
?
benzene + NADH + O2
-
Pseudomonas putida ML2 cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
?
benzene + NADH + O2 reaction in benzene catabolism Pseudomonas putida ?
-
?
benzene + NADH + O2 reaction in benzene catabolism Pseudomonas putida ML2 ?
-
?
trifluoromethylbenzene + NADH + O2
-
Pseudomonas putida ?
-
?
trifluoromethylbenzene + NADH + O2
-
Pseudomonas putida ML2 ?
-
?

Subunits

Subunits Comment Organism
? alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da Pseudomonas putida
More the benzene dioxygenase comprises three components: 1. flavoprotein, NADH:ferredoxin oxidoreductase, 2. an intermediate electron-transfer protein, or ferredoxin and 3. a terminal dioxygenase Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD contains FAD Pseudomonas putida
Ferredoxin the benzene dioxygenase comprises three components: 1. flavoprotein, NADH:ferredoxin oxidoreductase, 2. an intermediate electron-transfer protein, or ferredoxin and 3. a terminal dioxygenase Pseudomonas putida
NADH
-
Pseudomonas putida