KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00782 | - |
NADH | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the terminal dioxygenase component contains two Fe2+ ions per molecule in addition to two [2Fe-2S] iron-sulfur clusters | Pseudomonas putida | |
Iron | the terminal dioxygenase component contains two Fe2+ ions per molecule in addition to two [2Fe-2S] iron-sulfur clusters. The ferredoxin component contains one [2Fe-2S] cluster | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23500 | - |
alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da | Pseudomonas putida |
42000 | - |
alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da | Pseudomonas putida |
54500 | - |
alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene + NADH + O2 | Pseudomonas putida | reaction in benzene catabolism | ? | - |
? | |
benzene + NADH + O2 | Pseudomonas putida ML2 | reaction in benzene catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida ML2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, stable for 6 months to 1 year | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene + NADH + O2 | - |
Pseudomonas putida | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
benzene + NADH + O2 | - |
Pseudomonas putida ML2 | cis-cyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? | |
benzene + NADH + O2 | reaction in benzene catabolism | Pseudomonas putida | ? | - |
? | |
benzene + NADH + O2 | reaction in benzene catabolism | Pseudomonas putida ML2 | ? | - |
? | |
trifluoromethylbenzene + NADH + O2 | - |
Pseudomonas putida | ? | - |
? | |
trifluoromethylbenzene + NADH + O2 | - |
Pseudomonas putida ML2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | alpha2, beta2, 2 * 54500 + 2 * 23500, terminal dioxygenase component. 2 * 42000, reductase component. The ferredoxin component has a MW of 12300 Da | Pseudomonas putida |
More | the benzene dioxygenase comprises three components: 1. flavoprotein, NADH:ferredoxin oxidoreductase, 2. an intermediate electron-transfer protein, or ferredoxin and 3. a terminal dioxygenase | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains FAD | Pseudomonas putida | |
Ferredoxin | the benzene dioxygenase comprises three components: 1. flavoprotein, NADH:ferredoxin oxidoreductase, 2. an intermediate electron-transfer protein, or ferredoxin and 3. a terminal dioxygenase | Pseudomonas putida | |
NADH | - |
Pseudomonas putida |