Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS

Markus, A.; Krekel, D.; Lingens, F.; J. Biol. Chem. 261, 12883-12888 (1986)

Data extracted from this reference:

Metals/Ions

Commentary

Organism

Structure

Iron

component A is an iron-sulfur protein, contains 1.6-1.8 mol of iron per subunit and 1.6-1.9 mol of acid-labile sulfide

Pseudomonas sp.

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

3-chlorophenylacetate + NADH + O2 + H2O

10% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + Cl- + NAD+

-

-

-

?

3-chlorophenylacetate + NADH + O2 + H2O

10% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp. CBS 3

3,4-dihydroxyphenylacetate + Cl- + NAD+

-

-

-

?

4-bromophenylacetate + NADH + O2 + H2O

102% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + Br- + NAD+

-

-

-

?

4-bromophenylacetate + NADH + O2 + H2O

102% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp. CBS 3

3,4-dihydroxyphenylacetate + Br- + NAD+

-

-

-

?

4-chlorophenylacetate + NADH + O2 + H2O

-

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + Cl- + NAD(P)+

-

-

-

?

4-chlorophenylacetate + NADH + O2 + H2O

-

439042

Pseudomonas sp. CBS 3

3,4-dihydroxyphenylacetate + Cl- + NAD(P)+

-

-

-

?

4-fluorophenylacetate + NADH + O2 + H2O

30% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + F- + NAD+

-

-

-

?

4-fluorophenylacetate + NADH + O2 + H2O

30% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp. CBS 3

3,4-dihydroxyphenylacetate + F- + NAD+

-

-

-

?

4-hydroxyphenylacetate + NADH + O2 + H2O

4% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + OH- + NAD+

-

-

-

?

additional information

not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate

439042

Pseudomonas sp.

?

-

-

-

?

additional information

not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate

439042

Pseudomonas sp. CBS 3

?

-

-

-

?

phenylacetate + NADH + O2 + H2O

6% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

?

-

-

-

?

Cofactor

Commentary

Organism

Structure

NADH

-

Pseudomonas sp.

Cofactor

Commentary

Organism

Structure

NADH

-

Pseudomonas sp.

Metals/Ions

Commentary

Organism

Structure

Iron

component A is an iron-sulfur protein, contains 1.6-1.8 mol of iron per subunit and 1.6-1.9 mol of acid-labile sulfide

Pseudomonas sp.

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

3-chlorophenylacetate + NADH + O2 + H2O

10% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + Cl- + NAD+

-

-

-

?

3-chlorophenylacetate + NADH + O2 + H2O

10% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp. CBS 3

3,4-dihydroxyphenylacetate + Cl- + NAD+

-

-

-

?

4-bromophenylacetate + NADH + O2 + H2O

102% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + Br- + NAD+

-

-

-

?

4-bromophenylacetate + NADH + O2 + H2O

102% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp. CBS 3

3,4-dihydroxyphenylacetate + Br- + NAD+

-

-

-

?

4-chlorophenylacetate + NADH + O2 + H2O

-

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + Cl- + NAD(P)+

-

-

-

?

4-chlorophenylacetate + NADH + O2 + H2O

-

439042

Pseudomonas sp. CBS 3

3,4-dihydroxyphenylacetate + Cl- + NAD(P)+

-

-

-

?

4-fluorophenylacetate + NADH + O2 + H2O

30% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + F- + NAD+

-

-

-

?

4-fluorophenylacetate + NADH + O2 + H2O

30% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp. CBS 3

3,4-dihydroxyphenylacetate + F- + NAD+

-

-

-

?

4-hydroxyphenylacetate + NADH + O2 + H2O

4% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

3,4-dihydroxyphenylacetate + OH- + NAD+

-

-

-

?

additional information

not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate

439042

Pseudomonas sp.

?

-

-

-

?

additional information

not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate

439042

Pseudomonas sp. CBS 3

?

-

-

-

?

phenylacetate + NADH + O2 + H2O

6% of the activity of 4-chlorophenylacetate

439042

Pseudomonas sp.

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

439043

Schweizer

Purification and some properti ...

Pseudomonas sp., Pseudomonas sp. CBS 3

J. Biol. Chem.

262

9340-9346

1987

-

-

-

-

-

1

7

3

-

1

1

-

-

7

1

-

1

-

-

-

1

1

8

2

-

-

-

2

-

1

-

1

3

-

-

-

-

-

-

3

-

-

1

-

7

-

3

-

1

1

-

-

1

-

1

-

-

1

1

8

2

-

-

2

-

1

-

1

-

-

-

-

-

-

-

439042

Markus

Purification and some properti ...

Pseudomonas sp., Pseudomonas sp. CBS 3

J. Biol. Chem.

261

12883-12888

1986

-

-

-

-

-

-

-

-

-

1

2

-

-

6

-

-

1

-

-

-

1

-

12

2

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

2

-

-

-

-

1

-

-

1

-

12

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-