Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | component A is an iron-sulfur protein, contains 1.6-1.8 mol of iron per subunit and 1.6-1.9 mol of acid-labile sulfide | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration | Pseudomonas sp. |
144000 | - |
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, ultracentrifugation | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. CBS 3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
dioxygenase component | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.144 | - |
dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chlorophenylacetate + NADH + O2 + H2O | 10% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | 3,4-dihydroxyphenylacetate + Cl- + NAD+ | - |
? | |
3-chlorophenylacetate + NADH + O2 + H2O | 10% of the activity of 4-chlorophenylacetate | Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + Cl- + NAD+ | - |
? | |
4-bromophenylacetate + NADH + O2 + H2O | 102% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | 3,4-dihydroxyphenylacetate + Br- + NAD+ | - |
? | |
4-bromophenylacetate + NADH + O2 + H2O | 102% of the activity of 4-chlorophenylacetate | Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + Br- + NAD+ | - |
? | |
4-chlorophenylacetate + NADH + O2 + H2O | - |
Pseudomonas sp. | 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+ | - |
? | |
4-chlorophenylacetate + NADH + O2 + H2O | - |
Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+ | - |
? | |
4-fluorophenylacetate + NADH + O2 + H2O | 30% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | 3,4-dihydroxyphenylacetate + F- + NAD+ | - |
? | |
4-fluorophenylacetate + NADH + O2 + H2O | 30% of the activity of 4-chlorophenylacetate | Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + F- + NAD+ | - |
? | |
4-hydroxyphenylacetate + NADH + O2 + H2O | 4% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | 3,4-dihydroxyphenylacetate + OH- + NAD+ | - |
? | |
additional information | not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate | Pseudomonas sp. | ? | - |
? | |
additional information | not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate | Pseudomonas sp. CBS 3 | ? | - |
? | |
phenylacetate + NADH + O2 + H2O | 6% of the activity of 4-chlorophenylacetate | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase | Pseudomonas sp. |
trimer | 3 * 46000-52000, dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas sp. |