BRENDA - Enzyme Database
show all sequences of 1.14.12.9

Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS

Markus, A.; Krekel, D.; Lingens, F.; J. Biol. Chem. 261, 12883-12888 (1986) View publication on PubMed

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
component A is an iron-sulfur protein, contains 1.6-1.8 mol of iron per subunit and 1.6-1.9 mol of acid-labile sulfide
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration
Pseudomonas sp.
144000
-
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, ultracentrifugation
Pseudomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. CBS 3
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
dioxygenase component
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.144
-
dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-chlorophenylacetate + NADH + O2 + H2O
10% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + Cl- + NAD+
-
-
-
?
3-chlorophenylacetate + NADH + O2 + H2O
10% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + Cl- + NAD+
-
-
-
?
4-bromophenylacetate + NADH + O2 + H2O
102% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + Br- + NAD+
-
-
-
?
4-bromophenylacetate + NADH + O2 + H2O
102% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + Br- + NAD+
-
-
-
?
4-chlorophenylacetate + NADH + O2 + H2O
-
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
?
4-chlorophenylacetate + NADH + O2 + H2O
-
439042
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
?
4-fluorophenylacetate + NADH + O2 + H2O
30% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + F- + NAD+
-
-
-
?
4-fluorophenylacetate + NADH + O2 + H2O
30% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + F- + NAD+
-
-
-
?
4-hydroxyphenylacetate + NADH + O2 + H2O
4% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + OH- + NAD+
-
-
-
?
additional information
not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate
439042
Pseudomonas sp.
?
-
-
-
?
additional information
not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate
439042
Pseudomonas sp. CBS 3
?
-
-
-
?
phenylacetate + NADH + O2 + H2O
6% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase
Pseudomonas sp.
trimer
3 * 46000-52000, dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
component A is an iron-sulfur protein, contains 1.6-1.8 mol of iron per subunit and 1.6-1.9 mol of acid-labile sulfide
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration
Pseudomonas sp.
144000
-
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, ultracentrifugation
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
dioxygenase component
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.144
-
dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-chlorophenylacetate + NADH + O2 + H2O
10% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + Cl- + NAD+
-
-
-
?
3-chlorophenylacetate + NADH + O2 + H2O
10% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + Cl- + NAD+
-
-
-
?
4-bromophenylacetate + NADH + O2 + H2O
102% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + Br- + NAD+
-
-
-
?
4-bromophenylacetate + NADH + O2 + H2O
102% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + Br- + NAD+
-
-
-
?
4-chlorophenylacetate + NADH + O2 + H2O
-
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
?
4-chlorophenylacetate + NADH + O2 + H2O
-
439042
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
?
4-fluorophenylacetate + NADH + O2 + H2O
30% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + F- + NAD+
-
-
-
?
4-fluorophenylacetate + NADH + O2 + H2O
30% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + F- + NAD+
-
-
-
?
4-hydroxyphenylacetate + NADH + O2 + H2O
4% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
3,4-dihydroxyphenylacetate + OH- + NAD+
-
-
-
?
additional information
not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate
439042
Pseudomonas sp.
?
-
-
-
?
additional information
not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate
439042
Pseudomonas sp. CBS 3
?
-
-
-
?
phenylacetate + NADH + O2 + H2O
6% of the activity of 4-chlorophenylacetate
439042
Pseudomonas sp.
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase
Pseudomonas sp.
trimer
3 * 46000-52000, dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE
Pseudomonas sp.
Other publictions for EC 1.14.12.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
439043
Schweizer
Purification and some properti ...
Pseudomonas sp., Pseudomonas sp. CBS 3
J. Biol. Chem.
262
9340-9346
1987
-
-
-
-
-
1
7
3
-
1
1
-
-
7
1
-
1
-
-
-
1
1
8
2
-
-
-
2
-
1
-
1
3
-
-
-
-
-
-
3
-
-
1
-
7
-
3
-
1
1
-
-
1
-
1
-
-
1
1
8
2
-
-
2
-
1
-
1
-
-
-
-
-
-
-
439042
Markus
Purification and some properti ...
Pseudomonas sp., Pseudomonas sp. CBS 3
J. Biol. Chem.
261
12883-12888
1986
-
-
-
-
-
-
-
-
-
1
2
-
-
6
-
-
1
-
-
-
1
-
12
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
1
-
12
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-