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Literature summary for 1.14.12.9 extracted from

  • Markus, A.; Krekel, D.; Lingens, F.
    Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS (1986), J. Biol. Chem., 261, 12883-12888.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Iron component A is an iron-sulfur protein, contains 1.6-1.8 mol of iron per subunit and 1.6-1.9 mol of acid-labile sulfide Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration Pseudomonas sp.
144000
-
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, ultracentrifugation Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. CBS 3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
dioxygenase component Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.144
-
dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorophenylacetate + NADH + O2 + H2O 10% of the activity of 4-chlorophenylacetate Pseudomonas sp. 3,4-dihydroxyphenylacetate + Cl- + NAD+
-
?
3-chlorophenylacetate + NADH + O2 + H2O 10% of the activity of 4-chlorophenylacetate Pseudomonas sp. CBS 3 3,4-dihydroxyphenylacetate + Cl- + NAD+
-
?
4-bromophenylacetate + NADH + O2 + H2O 102% of the activity of 4-chlorophenylacetate Pseudomonas sp. 3,4-dihydroxyphenylacetate + Br- + NAD+
-
?
4-bromophenylacetate + NADH + O2 + H2O 102% of the activity of 4-chlorophenylacetate Pseudomonas sp. CBS 3 3,4-dihydroxyphenylacetate + Br- + NAD+
-
?
4-chlorophenylacetate + NADH + O2 + H2O
-
Pseudomonas sp. 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
?
4-chlorophenylacetate + NADH + O2 + H2O
-
Pseudomonas sp. CBS 3 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
?
4-fluorophenylacetate + NADH + O2 + H2O 30% of the activity of 4-chlorophenylacetate Pseudomonas sp. 3,4-dihydroxyphenylacetate + F- + NAD+
-
?
4-fluorophenylacetate + NADH + O2 + H2O 30% of the activity of 4-chlorophenylacetate Pseudomonas sp. CBS 3 3,4-dihydroxyphenylacetate + F- + NAD+
-
?
4-hydroxyphenylacetate + NADH + O2 + H2O 4% of the activity of 4-chlorophenylacetate Pseudomonas sp. 3,4-dihydroxyphenylacetate + OH- + NAD+
-
?
additional information not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate Pseudomonas sp. ?
-
?
additional information not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate Pseudomonas sp. CBS 3 ?
-
?
phenylacetate + NADH + O2 + H2O 6% of the activity of 4-chlorophenylacetate Pseudomonas sp. ?
-
?

Subunits

Subunits Comment Organism
More the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase Pseudomonas sp.
trimer 3 * 46000-52000, dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas sp.