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Literature summary for 1.14.13.1 extracted from

  • Zhao, H.; Chen, D.; Li, Y.; Cai, B.
    Overexpression, purification and characterization of a new salicylate hydroxylase from naphthalene-degrading Pseudomonas sp. strain ND6 (2005), Microbiol. Res., 160, 307-313.
    View publication on PubMed
Search for more literature for 1.14.13.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 salicylate enzyme form NahG Pseudomonas sp.
0.00494
-
 salicylate enzyme form NahU Pseudomonas sp.
0.02326
-
 NADH enzyme form NahG Pseudomonas sp.
0.07974
-
 NADH enzyme form NahU Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. ND6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA His Bind resin column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no activity of enzyme form NahU when using 3,5-dinitrosalicylate as substrate Pseudomonas sp.
1.27
-
 enzyme form NahG, using 3,5-dinitrosalicylate as substrate, at 20°C Pseudomonas sp.
1.53
-
 enzyme form NahU, using sulfosalicylate as substrate, at 20°C Pseudomonas sp.
3.17
-
 enzyme form NahG, using salicylate as substrate, at 20°C Pseudomonas sp.
3.63
-
 enzyme form NahG, using 5-chlorosalicylate as substrate, at 20°C Pseudomonas sp.
3.91
-
 enzyme form NahG, using 5-methylsalicylate as substrate, at 20°C Pseudomonas sp.
4.04
-
 enzyme form NahG, using 3-methylsalicylate as substrate, at 20°C Pseudomonas sp.
4.12
-
 enzyme form NahG, using aspirin as substrate, at 20°C Pseudomonas sp.
4.24
-
 enzyme form NahG, using sulfosalicylate as substrate, at 20°C Pseudomonas sp.
5.25
-
 enzyme form NahU, using 5-chlorosalicylate as substrate, at 20°C Pseudomonas sp.
10.59
-
 enzyme form NahU, using 3-methylsalicylate as substrate, at 20°C Pseudomonas sp.
18.79
-
 enzyme form NahU, using 5-methylsalicylate as substrate, at 20°C Pseudomonas sp.
23.44
-
 enzyme form NahU, using salicylate as substrate, at 20°C Pseudomonas sp.
25.64
-
 enzyme form NahU, using aspirin as substrate, at 20°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-dinitrosalicylate + NADH + O2
-
 Pseudomonas sp. ? + NAD+ + H2O + CO2
-
 ?
3,5-dinitrosalicylate + NADH + O2
-
 Pseudomonas sp. ND6 ? + NAD+ + H2O + CO2
-
 ?
3-methylsalicylate + NADH + O2
-
 Pseudomonas sp. 1,2-dihydroxy-3-methylbenzene + NAD+ + H2O + CO2
-
 ?
3-methylsalicylate + NADH + O2
-
 Pseudomonas sp. ND6 1,2-dihydroxy-3-methylbenzene + NAD+ + H2O + CO2
-
 ?
5-chlorosalicylate + NADH + O2
-
 Pseudomonas sp. 1,2-dihydroxy-5-chlorobenzene + NAD+ + H2O + CO2
-
 ?
5-chlorosalicylate + NADH + O2
-
 Pseudomonas sp. ND6 1,2-dihydroxy-5-chlorobenzene + NAD+ + H2O + CO2
-
 ?
5-methylsalicylate + NADH + H+ + O2
-
 Pseudomonas sp. 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O
-
 ?
5-methylsalicylate + NADH + H+ + O2
-
 Pseudomonas sp. ND6 1,2-dihydroxy-5-methylbenzene + CO2 + NAD+ + H2O
-
 ?
aspirin + NADH + O2
-
 Pseudomonas sp. ? + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas sp. catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas sp. ND6 catechol + NAD+ + H2O + CO2
-
 ?
sulfosalicylate + NADH + O2
-
 Pseudomonas sp. ? + NAD+ + H2O + CO2
-
 ?

Synonyms

Synonyms Comment Organism
NahG
-
 Pseudomonas sp.
NahU
-
 Pseudomonas sp.
salicylate hydroxylase
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas sp.
NADH
-
 Pseudomonas sp.