BRENDA - Enzyme Database
show all sequences of 1.14.13.101

The two faces of pyrrolizidine alkaloids: the role of the tertiary amine and its N-oxide in chemical defense of insects with aquired alkaloids

Lindigkeit, R.; Biller, A.; Buch, M.; Schiebel, H.M.; Boppre, M.; Hartmann, T.; Eur. J. Biochem. 245, 626-636 (1997)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
NADPH
pH 7.0, pH 37°C
Tyria jacobaeae
0.0014
-
senecionine
pH 7.0, 37°C
Tyria jacobaeae
0.0029
-
monocrotaline
pH 7.0, 37°C
Creatonotos transiens
0.0031
-
senecionine
pH 7.0, 37°C
Creatonotos transiens
0.0048
-
senecionine
pH 7.0, 37°C
Arctia caja
0.0051
-
monocrotaline
pH 7.0, 37°C
Arctia caja
0.0125
-
monocrotaline
pH 7.0, 37°C
Tyria jacobaeae
0.0186
-
heliotrine
pH 7.0, 37°C
Arctia caja
0.0229
-
heliotrine
pH 7.0, 37°C
Creatonotos transiens
0.284
-
heliotrine
pH 7.0, 37°C
Tyria jacobaeae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Tyria jacobaeae
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51000
-
4 * 51000, SDS-PAGE
Tyria jacobaeae
200000
-
gel filtration
Tyria jacobaeae
Organism
Organism
UniProt
Commentary
Textmining
Amerila phaedra
-
-
-
Arctia caja
-
-
-
Arctia intercalaris
-
-
-
Callimorpha dominula
-
-
-
Creatonotos transiens
-
-
-
Idea leuconoe
-
-
-
no activity in Spodoptera littoralis
-
-
-
Tyria jacobaeae
-
-
-
Zonocerus variegatus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Tyria jacobaeae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adult
-
Amerila phaedra
-
fat body
-
Zonocerus variegatus
-
hemolymph
from late instar larvae
Tyria jacobaeae
-
larva
-
Arctia caja
-
larva
-
Creatonotos transiens
-
larva
-
Callimorpha dominula
-
larva
-
Arctia intercalaris
-
pupa
-
Idea leuconoe
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.67
-
-
Tyria jacobaeae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
axillaridine + NADPH + O2
76% of the activity with senecionine
651040
Tyria jacobaeae
axillaridine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
axillaridine + NADPH + O2
79% of the activity with senecionine
651040
Arctia caja
axillaridine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
axillaridine + NADPH + O2
93% of the activity with senecionine
651040
Creatonotos transiens
axillaridine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
axillarine + NADPH + O2
38% of the activity with senecionine
651040
Creatonotos transiens
axillarine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
axillarine + NADPH + O2
55% of the activity with senecionine
651040
Arctia caja
axillarine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
axillarine + NADPH + O2
74% of the activity with senecionine
651040
Tyria jacobaeae
axillarine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
heliotrine + NADPH + O2
-
651040
Tyria jacobaeae
heliotrine N-oxide + NADP+ + H2O
-
-
-
?
heliotrine + NADPH + O2
115% of the activity with senecionine
651040
Creatonotos transiens
heliotrine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
heliotrine + NADPH + O2
25% of the activity with senecionine
651040
Tyria jacobaeae
heliotrine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
heliotrine + NADPH + O2
86% of the activity with senecionine
651040
Arctia caja
heliotrine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
indicine + NADPH + O2
35% of the activity with senecionine
651040
Tyria jacobaeae
indicine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
indicine + NADPH + O2
78% of the activity with senecionine
651040
Creatonotos transiens
indicine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
indicine + NADPH + O2
90% of the activity with senecionine
651040
Arctia caja
indicine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
lycopsamine + NADPH + O2
20% of the activity with senecionine
651040
Tyria jacobaeae
lycopsamine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
lycopsamine + NADPH + O2
75% of the activity with senecionine
651040
Creatonotos transiens
lycopsamine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
lycopsamine + NADPH + O2
93% of the activity with senecionine
651040
Arctia caja
lycopsamine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
monocrotaline + NADPH + O2
-
651040
Tyria jacobaeae
monocrotaline N-oxide + NADP+ + H2O
-
-
-
?
monocrotaline + NADPH + O2
103% of the activity with senecionine
651040
Arctia caja
monocrotaline N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
monocrotaline + NADPH + O2
110% of the activity with senecionine
651040
Creatonotos transiens
monocrotaline N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
monocrotaline + NADPH + O2
92% of the activity with senecionine
651040
Tyria jacobaeae
monocrotaline N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
additional information
the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines
651040
Tyria jacobaeae
?
-
651040
Tyria jacobaeae
?
additional information
the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: triangularine, senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines
651040
Arctia caja
?
-
651040
Arctia caja
?
additional information
the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: triangularine, senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines
651040
Creatonotos transiens
?
-
651040
Creatonotos transiens
?
retrorsine + NADPH + O2
93% of the activity with senecionine
651040
Creatonotos transiens
retrorsine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
retrorsine + NADPH + O2
97% of the activity with senecionine
651040
Tyria jacobaeae
retrorsine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
retrorsine + NADPH + O2
as active as senecionine
651040
Arctia caja
retrorsine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
rinderine + NADPH + O2
23% of the activity with senecionine
651040
Tyria jacobaeae
rinderine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
rinderine + NADPH + O2
79% of the activity with senecionine
651040
Arctia caja
rinderine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
rinderine + NADPH + O2
81% of the activity with senecionine
651040
Creatonotos transiens
rinderine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
senecionine + NADPH + O2
-
651040
Arctia caja
senecionine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
senecionine + NADPH + O2
-
651040
Tyria jacobaeae
senecionine N-oxide + NADP+ + H2O
-
-
-
?
senecionine + NADPH + O2
-
651040
Tyria jacobaeae
senecionine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
senecionine + NADPH + O2
-
651040
Creatonotos transiens
senecionine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
seneciphylline + NADPH + O2
117% of the activity with senecionine
651040
Arctia caja
seneciphylline N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
seneciphylline + NADPH + O2
119% of the activity with senecionine
651040
Creatonotos transiens
seneciphylline N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
seneciphylline + NADPH + O2
95% of the activity with senecionine
651040
Tyria jacobaeae
seneciphylline N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
senecivernine + NADPH + O2
107% of the activity with senecionine
651040
Arctia caja
senecivernine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
senecivernine + NADPH + O2
113% of the activity with senecionine
651040
Creatonotos transiens
senecivernine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
senecivernine + NADPH + O2
81% of the activity with senecionine
651040
Tyria jacobaeae
senecivernine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
triangularine + NADPH + O2
60% of the activity with senecionine
651040
Tyria jacobaeae
triangularine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 51000, SDS-PAGE
Tyria jacobaeae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
45
-
Tyria jacobaeae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Tyria jacobaeae
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
molar apoenzyme/FAD ratio of 0.4
Tyria jacobaeae
NADPH
strictly dependent on
Arctia caja
NADPH
strictly dependent on
Creatonotos transiens
NADPH
strictly dependent on, no reaction with NADH
Tyria jacobaeae
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Tyria jacobaeae
determination on an Ampholine PAGplate pH 3.5-9.5 using a horizantal electrophoresis system
-
4.9
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
molar apoenzyme/FAD ratio of 0.4
Tyria jacobaeae
NADPH
strictly dependent on
Arctia caja
NADPH
strictly dependent on
Creatonotos transiens
NADPH
strictly dependent on, no reaction with NADH
Tyria jacobaeae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0013
-
NADPH
pH 7.0, pH 37°C
Tyria jacobaeae
0.0014
-
senecionine
pH 7.0, 37°C
Tyria jacobaeae
0.0029
-
monocrotaline
pH 7.0, 37°C
Creatonotos transiens
0.0031
-
senecionine
pH 7.0, 37°C
Creatonotos transiens
0.0048
-
senecionine
pH 7.0, 37°C
Arctia caja
0.0051
-
monocrotaline
pH 7.0, 37°C
Arctia caja
0.0125
-
monocrotaline
pH 7.0, 37°C
Tyria jacobaeae
0.0186
-
heliotrine
pH 7.0, 37°C
Arctia caja
0.0229
-
heliotrine
pH 7.0, 37°C
Creatonotos transiens
0.284
-
heliotrine
pH 7.0, 37°C
Tyria jacobaeae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Tyria jacobaeae
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51000
-
4 * 51000, SDS-PAGE
Tyria jacobaeae
200000
-
gel filtration
Tyria jacobaeae
Purification (Commentary) (protein specific)
Commentary
Organism
-
Tyria jacobaeae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adult
-
Amerila phaedra
-
fat body
-
Zonocerus variegatus
-
hemolymph
from late instar larvae
Tyria jacobaeae
-
larva
-
Arctia caja
-
larva
-
Creatonotos transiens
-
larva
-
Callimorpha dominula
-
larva
-
Arctia intercalaris
-
pupa
-
Idea leuconoe
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.67
-
-
Tyria jacobaeae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
axillaridine + NADPH + O2
76% of the activity with senecionine
651040
Tyria jacobaeae
axillaridine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
axillaridine + NADPH + O2
79% of the activity with senecionine
651040
Arctia caja
axillaridine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
axillaridine + NADPH + O2
93% of the activity with senecionine
651040
Creatonotos transiens
axillaridine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
axillarine + NADPH + O2
38% of the activity with senecionine
651040
Creatonotos transiens
axillarine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
axillarine + NADPH + O2
55% of the activity with senecionine
651040
Arctia caja
axillarine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
axillarine + NADPH + O2
74% of the activity with senecionine
651040
Tyria jacobaeae
axillarine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
heliotrine + NADPH + O2
-
651040
Tyria jacobaeae
heliotrine N-oxide + NADP+ + H2O
-
-
-
?
heliotrine + NADPH + O2
115% of the activity with senecionine
651040
Creatonotos transiens
heliotrine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
heliotrine + NADPH + O2
25% of the activity with senecionine
651040
Tyria jacobaeae
heliotrine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
heliotrine + NADPH + O2
86% of the activity with senecionine
651040
Arctia caja
heliotrine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
indicine + NADPH + O2
35% of the activity with senecionine
651040
Tyria jacobaeae
indicine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
indicine + NADPH + O2
78% of the activity with senecionine
651040
Creatonotos transiens
indicine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
indicine + NADPH + O2
90% of the activity with senecionine
651040
Arctia caja
indicine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
lycopsamine + NADPH + O2
20% of the activity with senecionine
651040
Tyria jacobaeae
lycopsamine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
lycopsamine + NADPH + O2
75% of the activity with senecionine
651040
Creatonotos transiens
lycopsamine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
lycopsamine + NADPH + O2
93% of the activity with senecionine
651040
Arctia caja
lycopsamine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
monocrotaline + NADPH + O2
-
651040
Tyria jacobaeae
monocrotaline N-oxide + NADP+ + H2O
-
-
-
?
monocrotaline + NADPH + O2
103% of the activity with senecionine
651040
Arctia caja
monocrotaline N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
monocrotaline + NADPH + O2
110% of the activity with senecionine
651040
Creatonotos transiens
monocrotaline N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
monocrotaline + NADPH + O2
92% of the activity with senecionine
651040
Tyria jacobaeae
monocrotaline N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
additional information
the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines
651040
Tyria jacobaeae
?
-
651040
Tyria jacobaeae
?
additional information
the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: triangularine, senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines
651040
Arctia caja
?
-
651040
Arctia caja
?
additional information
the enzyme N-oxidizes only alkaloids with structural elements which are essential for hepatotoxic and genotoxic pyrrolizidine alkaloids, i.e. 1,2-double bond, esterification of the allylic hydroxyl group, presence of a second free or esterified hydroxyl group at carbon 7. No activity with: triangularine, senkirkine, sarracine, supinine, phalaenopsine, retronecine, heliotridine, supinidine, isoretronecanol, nicotine, caffeine, tropane alkaloids, quinolizidine alkaloids, indole alkaloids, isoquinolines and synthetic heterocyclic tertiary amines
651040
Creatonotos transiens
?
-
651040
Creatonotos transiens
?
retrorsine + NADPH + O2
93% of the activity with senecionine
651040
Creatonotos transiens
retrorsine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
retrorsine + NADPH + O2
97% of the activity with senecionine
651040
Tyria jacobaeae
retrorsine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
retrorsine + NADPH + O2
as active as senecionine
651040
Arctia caja
retrorsine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
rinderine + NADPH + O2
23% of the activity with senecionine
651040
Tyria jacobaeae
rinderine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
rinderine + NADPH + O2
79% of the activity with senecionine
651040
Arctia caja
rinderine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
rinderine + NADPH + O2
81% of the activity with senecionine
651040
Creatonotos transiens
rinderine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
senecionine + NADPH + O2
-
651040
Arctia caja
senecionine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
senecionine + NADPH + O2
-
651040
Tyria jacobaeae
senecionine N-oxide + NADP+ + H2O
-
-
-
?
senecionine + NADPH + O2
-
651040
Tyria jacobaeae
senecionine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
senecionine + NADPH + O2
-
651040
Creatonotos transiens
senecionine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
seneciphylline + NADPH + O2
117% of the activity with senecionine
651040
Arctia caja
seneciphylline N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
seneciphylline + NADPH + O2
119% of the activity with senecionine
651040
Creatonotos transiens
seneciphylline N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
seneciphylline + NADPH + O2
95% of the activity with senecionine
651040
Tyria jacobaeae
seneciphylline N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
senecivernine + NADPH + O2
107% of the activity with senecionine
651040
Arctia caja
senecivernine N-oxide + NADP+ + H2O
-
651040
Arctia caja
?
senecivernine + NADPH + O2
113% of the activity with senecionine
651040
Creatonotos transiens
senecivernine N-oxide + NADP+ + H2O
-
651040
Creatonotos transiens
?
senecivernine + NADPH + O2
81% of the activity with senecionine
651040
Tyria jacobaeae
senecivernine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
triangularine + NADPH + O2
60% of the activity with senecionine
651040
Tyria jacobaeae
triangularine N-oxide + NADP+ + H2O
-
651040
Tyria jacobaeae
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 51000, SDS-PAGE
Tyria jacobaeae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
45
-
Tyria jacobaeae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Tyria jacobaeae
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Tyria jacobaeae
determination on an Ampholine PAGplate pH 3.5-9.5 using a horizantal electrophoresis system
-
4.9
Other publictions for EC 1.14.13.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728567
Wang
Independent recruitment of a f ...
Zonocerus variegatus
PLoS ONE
7
e31796
2012
-
-
1
-
-
-
-
5
-
-
2
2
-
1
-
-
1
-
-
-
-
-
17
1
2
1
-
-
5
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
2
2
-
-
-
1
-
-
-
-
17
1
1
-
-
5
1
-
-
-
-
-
-
-
5
5
706452
Sehlmeyer
Flavin-dependent monooxygenase ...
Apantesis nevadensis geneura, Tyria jacobaeae
PLoS ONE
5
e10435
2010
-
-
2
-
-
-
-
-
-
-
-
1
-
11
-
-
-
-
-
2
1
-
10
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
653688
Naumann
Evolutionary recruitment of a ...
Tyria jacobaeae
Proc. natl. Acad. Sci. USA
99
6085-6090
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
11
-
-
-
-
-
1
-
-
7
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689412
Chang
-
Solubilization and characteriz ...
Crotalaria scassellatii
Phytochemistry
49
1859-1866
1998
3
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
1
-
1
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
651040
Lindigkeit
The two faces of pyrrolizidine ...
Arctia caja, Tyria jacobaeae, no activity in Spodoptera littoralis, Creatonotos transiens, Callimorpha dominula, Arctia intercalaris, Amerila phaedra, Idea leuconoe, Zonocerus variegatus
Eur. J. Biochem.
245
626-636
1997
-
-
-
-
-
-
-
10
1
-
2
-
-
17
-
-
1
-
-
8
1
-
40
1
-
1
-
-
-
1
-
-
4
-
1
-
-
-
-
4
-
-
-
-
-
-
10
1
-
2
-
-
-
-
1
-
8
1
-
40
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-