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show all sequences of 1.14.13.130

Purification and characterization of a pyrrole-2-carboxylate oxygenase from Arthrobacter strain Py

Hormann, K; Andreesen, J.R.; Biol. Chem. Hoppe-Seyler 375, 211-218 (1994)

Data extracted from this reference:

General Stability
General Stability
Organism
FAD and dithioerythritol stabilize enzyme activity during purification
Arthrobacter sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoic acid)
0.5 mM, 87% loss of activity
Arthrobacter sp.
Cu+
0.001-0.01 mM, 93% loss of activity
Arthrobacter sp.
Hg2+
0.01-0.1 mM, 96% loss of activity
Arthrobacter sp.
additional information
no inhibition by 1 mM EDTA or 1 mM arsenite
Arthrobacter sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Arthrobacter sp.
160000
-
gel filtration
Arthrobacter sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyrrole-2-carboxylate + NADH + H+ + O2
Arthrobacter sp.
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
?
pyrrole-2-carboxylate + NADH + H+ + O2
Arthrobacter sp. Py1
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. Py1
-
-
-
Purification (Commentary)
Commentary
Organism
FAD and dithioerythritol stabilize enzyme activity during purification
Arthrobacter sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:pyrrole-2-carboxylate-grown cell
-
Arthrobacter sp.
-
Storage Stability
Storage Stability
Organism
-20C, several months, stable
Arthrobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyrrole-2-carboxylate + NADH + H+ + O2
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
707605
Arthrobacter sp.
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
-
?
pyrrole-2-carboxylate + NADH + H+ + O2
no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate
707605
Arthrobacter sp.
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
-
?
pyrrole-2-carboxylate + NADH + H+ + O2
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
707605
Arthrobacter sp. Py1
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
-
?
pyrrole-2-carboxylate + NADH + H+ + O2
no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate
707605
Arthrobacter sp. Py1
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Arthrobacter sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
9
-
Arthrobacter sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.2
9.5
pH 7.2: 65% of maximal activity, pH 9.5: 68% of maximal activity
Arthrobacter sp.
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein. Contains 2.7-3.6 mol of FAD per mol of enzyme
Arthrobacter sp.
NADH
no activity with NADPH
Arthrobacter sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Arthrobacter sp.
-
-
4.6
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein. Contains 2.7-3.6 mol of FAD per mol of enzyme
Arthrobacter sp.
NADH
no activity with NADPH
Arthrobacter sp.
General Stability (protein specific)
General Stability
Organism
FAD and dithioerythritol stabilize enzyme activity during purification
Arthrobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoic acid)
0.5 mM, 87% loss of activity
Arthrobacter sp.
Cu+
0.001-0.01 mM, 93% loss of activity
Arthrobacter sp.
Hg2+
0.01-0.1 mM, 96% loss of activity
Arthrobacter sp.
additional information
no inhibition by 1 mM EDTA or 1 mM arsenite
Arthrobacter sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Arthrobacter sp.
160000
-
gel filtration
Arthrobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyrrole-2-carboxylate + NADH + H+ + O2
Arthrobacter sp.
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
?
pyrrole-2-carboxylate + NADH + H+ + O2
Arthrobacter sp. Py1
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
FAD and dithioerythritol stabilize enzyme activity during purification
Arthrobacter sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:pyrrole-2-carboxylate-grown cell
-
Arthrobacter sp.
-
Storage Stability (protein specific)
Storage Stability
Organism
-20C, several months, stable
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyrrole-2-carboxylate + NADH + H+ + O2
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
707605
Arthrobacter sp.
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
-
?
pyrrole-2-carboxylate + NADH + H+ + O2
no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate
707605
Arthrobacter sp.
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
-
?
pyrrole-2-carboxylate + NADH + H+ + O2
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
707605
Arthrobacter sp. Py1
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
-
?
pyrrole-2-carboxylate + NADH + H+ + O2
no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate
707605
Arthrobacter sp. Py1
5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Arthrobacter sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
9
-
Arthrobacter sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.2
9.5
pH 7.2: 65% of maximal activity, pH 9.5: 68% of maximal activity
Arthrobacter sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Arthrobacter sp.
-
-
4.6
Expression
Organism
Commentary
Expression
Arthrobacter sp.
activity is induced by wrowth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
up
General Information
General Information
Commentary
Organism
physiological function
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
Arthrobacter sp.
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
Arthrobacter sp.
Expression (protein specific)
Organism
Commentary
Expression
Arthrobacter sp.
activity is induced by wrowth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy
up
Other publictions for EC 1.14.13.130
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
708366
Becker
Two-component flavin-dependent ...
Rhodococcus sp.
Eur. J. Biochem.
249
739-747
1997
-
-
-
-
-
-
4
3
-
3
2
1
-
3
-
-
1
-
-
1
1
-
3
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
3
-
3
2
1
-
-
-
1
-
1
1
-
3
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
707605
Hormann
Purification and characterizat ...
Arthrobacter sp., Arthrobacter sp. Py1
Biol. Chem. Hoppe-Seyler
375
211-218
1994
-
-
-
-
-
1
4
-
-
-
2
2
-
2
-
-
1
-
-
1
-
1
4
1
-
-
-
-
1
1
-
2
-
1
-
-
-
-
2
-
-
1
-
4
-
-
-
-
2
2
-
-
-
1
-
1
-
1
4
1
-
-
-
-
1
1
-
1
1
1
1
1
-
-