General Stability | Organism |
---|---|
FAD and dithioerythritol stabilize enzyme activity during purification | Arthrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 0.5 mM, 87% loss of activity | Arthrobacter sp. | |
Cu+ | 0.001-0.01 mM, 93% loss of activity | Arthrobacter sp. | |
Hg2+ | 0.01-0.1 mM, 96% loss of activity | Arthrobacter sp. | |
additional information | no inhibition by 1 mM EDTA or 1 mM arsenite | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Arthrobacter sp. |
160000 | - |
gel filtration | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyrrole-2-carboxylate + NADH + H+ + O2 | Arthrobacter sp. | the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy | 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O | - |
? | |
pyrrole-2-carboxylate + NADH + H+ + O2 | Arthrobacter sp. Py1 | the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy | 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Arthrobacter sp. Py1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
FAD and dithioerythritol stabilize enzyme activity during purification | Arthrobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:pyrrole-2-carboxylate-grown cell | - |
Arthrobacter sp. | - |
Storage Stability | Organism |
---|---|
-20°C, several months, stable | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyrrole-2-carboxylate + NADH + H+ + O2 | the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy | Arthrobacter sp. | 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O | - |
? | |
pyrrole-2-carboxylate + NADH + H+ + O2 | no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate | Arthrobacter sp. | 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O | - |
? | |
pyrrole-2-carboxylate + NADH + H+ + O2 | the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy | Arthrobacter sp. Py1 | 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O | - |
? | |
pyrrole-2-carboxylate + NADH + H+ + O2 | no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate | Arthrobacter sp. Py1 | 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.5 | pH 7.2: 65% of maximal activity, pH 9.5: 68% of maximal activity | Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein. Contains 2.7-3.6 mol of FAD per mol of enzyme | Arthrobacter sp. | |
NADH | no activity with NADPH | Arthrobacter sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arthrobacter sp. | - |
- |
4.6 |
Organism | Comment | Expression |
---|---|---|
Arthrobacter sp. | activity is induced by wrowth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy | Arthrobacter sp. |