BRENDA - Enzyme Database
show all sequences of 1.14.13.14

Phenylalanine ammonia lyase and cinnamic acid hydroxylases as assembled consecutive enzymes on microsomal membranes of cucumber cotyledons: cooperation and subcellular distribution

Czichi, U.; Kindl, H.; Planta 134, 133-143 (1977)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
white light treatment for 200 min leads to 50% activation
Cucumis sativus
sulfonic acid
700% activation at 0.003%
Cucumis sativus
Triton X-100
700% activation at 0.025%
Cucumis sativus
Inhibitors
Inhibitors
Commentary
Organism
Structure
KCN
70% inhibition at 10 mM
Cucumis sativus
additional information
UV-light treatment for 200 min leads to 30% inhibition
Cucumis sativus
Triton X-100
concentration higher than 0.5% deactivates the enzyme
Cucumis sativus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
predominantly, chloroplast membrane
Cucumis sativus
9507
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trans-cinnamate + NADPH + O2
Cucumis sativus
-
2-hydroxycinnamate + NADP+ + H2O
-
Cucumis sativus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cucumis sativus
-
cucumber
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Cucumis sativus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trans-cinnamate + NADPH + O2
-
439037
Cucumis sativus
2-hydroxycinnamate + NADP+ + H2O
-
439037
Cucumis sativus
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Cucumis sativus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
white light treatment for 200 min leads to 50% activation
Cucumis sativus
sulfonic acid
700% activation at 0.003%
Cucumis sativus
Triton X-100
700% activation at 0.025%
Cucumis sativus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Cucumis sativus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
KCN
70% inhibition at 10 mM
Cucumis sativus
additional information
UV-light treatment for 200 min leads to 30% inhibition
Cucumis sativus
Triton X-100
concentration higher than 0.5% deactivates the enzyme
Cucumis sativus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
predominantly, chloroplast membrane
Cucumis sativus
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trans-cinnamate + NADPH + O2
Cucumis sativus
-
2-hydroxycinnamate + NADP+ + H2O
-
Cucumis sativus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Cucumis sativus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trans-cinnamate + NADPH + O2
-
439037
Cucumis sativus
2-hydroxycinnamate + NADP+ + H2O
-
439037
Cucumis sativus
?
Other publictions for EC 1.14.13.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
439037
Czichi
Phenylalanine ammonia lyase an ...
Cucumis sativus
Planta
134
133-143
1977
3
-
-
-
-
-
3
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
1
-
-
-
-
3
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439038
Gestetner
The 2-hydroxylation of trans-c ...
Melilotus albus
Arch. Biochem. Biophys.
163
617-624
1974
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-