BRENDA - Enzyme Database
show all sequences of 1.14.13.14

The 2-hydroxylation of trans-cinnamic acid by chloroplasts from Melilotus alba Desr

Gestetner, B.; Conn, E.E.; Arch. Biochem. Biophys. 163, 617-624 (1974)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
glucose 6-phosphate
400% activation at 4 mM
Melilotus albus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
lamellar, 50% of the hydroxylase activity
Melilotus albus
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trans-cinnamate + NADPH + O2
Melilotus albus
-
2-hydroxycinnamate + NADP+ + H2O
-
Melilotus albus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Melilotus albus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Melilotus albus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0004
-
supernatant activity
Melilotus albus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trans-cinnamate + NADPH + O2
-
439038
Melilotus albus
2-hydroxycinnamate + NADP+ + H2O
-
439038
Melilotus albus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Melilotus albus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
less effective than NADPH
Melilotus albus
NADPH
-
Melilotus albus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
glucose 6-phosphate
400% activation at 4 mM
Melilotus albus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
less effective than NADPH
Melilotus albus
NADPH
-
Melilotus albus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
lamellar, 50% of the hydroxylase activity
Melilotus albus
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
trans-cinnamate + NADPH + O2
Melilotus albus
-
2-hydroxycinnamate + NADP+ + H2O
-
Melilotus albus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Melilotus albus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0004
-
supernatant activity
Melilotus albus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
trans-cinnamate + NADPH + O2
-
439038
Melilotus albus
2-hydroxycinnamate + NADP+ + H2O
-
439038
Melilotus albus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Melilotus albus
Other publictions for EC 1.14.13.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
439037
Czichi
Phenylalanine ammonia lyase an ...
Cucumis sativus
Planta
134
133-143
1977
3
-
-
-
-
-
3
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
1
-
-
-
-
3
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439038
Gestetner
The 2-hydroxylation of trans-c ...
Melilotus albus
Arch. Biochem. Biophys.
163
617-624
1974
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-