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Literature summary for 1.14.13.149 extracted from

  • Grishin, A.; Ajamian, E.; Zhang, L.; Cygler, M.
    Crystallization and preliminary X-ray analysis of PaaAC, the main component of the hydroxylase of the Escherichia coli phenylacetyl-coenzyme A oxygenase complex (2010), Acta Crystallogr. Sect. F, 66, 1045-1049.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.149

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, in the absence of a bound ligand (with 100 mM N-(2-acetamido)-iminodiacetic acid pH 5.5) as well as in complexes with CoA, 3-hydroxybutyryl-CoA, benzoyl-CoA and phenylacetyl-CoA, using either 0.1 M PIPES pH 6.5, 15% (w/v) PEG 550 monomethyl ether or 0.1 M PIPES pH 6.5, 5% (v/v) 2-propanol, 5% (w/v) PEG 550 monomethyl ether Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme shows low affinity for iron, some rearrangement of the protein is induced by iron binding Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superose 12 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylacetyl-CoA + O2 + NADPH + H+ true substrate Escherichia coli 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + H2O + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
PaaAC
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Escherichia coli