BRENDA - Enzyme Database show
show all sequences of 1.14.13.158

Molecular cloning of an aldehyde dehydrogenase implicated in artemisinin biosynthesis in Artemisia annua

Teoh, K.; Polichuk, D.; Reed, D.; Covello, P.; Botany 87, 635-642 (2009)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Artemisia annua
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
artemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
0.0088
-
dihydroartemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53800
-
x * 53800, calculated
Artemisia annua
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Artemisia annua
-
-
-
Artemisia annua
C5I9X1
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
trichome
glandular secretory trichome
Artemisia annua
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-nonenal + O2 + NADPH + H+
-
717445
Artemisia annua
? + NADP+ + 2 H2O
-
-
-
-
amorpha-4,11-diene + O2 + NADPH + H+
-
717445
Artemisia annua
artemisinic alcohol + NADP+ + H2O
-
-
-
-
artemisinic alcohol + O2 + NADPH + H+
-
717445
Artemisia annua
artemisinic aldehyde + NADP+ + 2 H2O
-
-
-
-
artemisinic aldehyde + O2 + NADPH + H+
-
717445
Artemisia annua
artemisinate + NADP+ + H2O
-
-
-
-
dihydroartemisinic alcohol + O2 + NADPH + H+
-
717445
Artemisia annua
dihydroartemisinic aldehyde + NADP+ + 2 H2O
-
-
-
-
dihydroartemisinic alcohol + O2 + NADPH + H+
about 50% of the rate with artemisinic alcohol
717445
Artemisia annua
dihydroartemisinic aldehyde + NADP+ + 2 H2O
-
-
-
-
dihydroartemisinic aldehyde + O2 + NADPH + H+
-
717445
Artemisia annua
dihydroartemisinate + NADP+ + H2O
-
-
-
-
additional information
no substrate: dihydroartemisinic aldehyde
717445
Artemisia annua
?
-
-
-
-
additional information
no substrates: Sinapaldehyde, 2-phenylpropanal
717445
Artemisia annua
?
-
-
-
-
octanal + O2 + NADPH + H+
-
717445
Artemisia annua
? + NADP+ + 2 H2O
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 53800, calculated
Artemisia annua
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.53
-
artemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
7.74
-
dihydroartemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
substrate dihydroartemisinic aldehyde
Artemisia annua
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADH is as effective as NADPH
Artemisia annua
NADPH
NADH is as effective as NADPH
Artemisia annua
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Artemisia annua
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADH is as effective as NADPH
Artemisia annua
NADPH
NADH is as effective as NADPH
Artemisia annua
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
artemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
0.0088
-
dihydroartemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53800
-
x * 53800, calculated
Artemisia annua
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
trichome
glandular secretory trichome
Artemisia annua
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-nonenal + O2 + NADPH + H+
-
717445
Artemisia annua
? + NADP+ + 2 H2O
-
-
-
-
amorpha-4,11-diene + O2 + NADPH + H+
-
717445
Artemisia annua
artemisinic alcohol + NADP+ + H2O
-
-
-
-
artemisinic alcohol + O2 + NADPH + H+
-
717445
Artemisia annua
artemisinic aldehyde + NADP+ + 2 H2O
-
-
-
-
artemisinic aldehyde + O2 + NADPH + H+
-
717445
Artemisia annua
artemisinate + NADP+ + H2O
-
-
-
-
dihydroartemisinic alcohol + O2 + NADPH + H+
-
717445
Artemisia annua
dihydroartemisinic aldehyde + NADP+ + 2 H2O
-
-
-
-
dihydroartemisinic alcohol + O2 + NADPH + H+
about 50% of the rate with artemisinic alcohol
717445
Artemisia annua
dihydroartemisinic aldehyde + NADP+ + 2 H2O
-
-
-
-
dihydroartemisinic aldehyde + O2 + NADPH + H+
-
717445
Artemisia annua
dihydroartemisinate + NADP+ + H2O
-
-
-
-
additional information
no substrate: dihydroartemisinic aldehyde
717445
Artemisia annua
?
-
-
-
-
additional information
no substrates: Sinapaldehyde, 2-phenylpropanal
717445
Artemisia annua
?
-
-
-
-
octanal + O2 + NADPH + H+
-
717445
Artemisia annua
? + NADP+ + 2 H2O
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 53800, calculated
Artemisia annua
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.53
-
artemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
7.74
-
dihydroartemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
substrate dihydroartemisinic aldehyde
Artemisia annua
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
593
-
artemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
881
-
dihydroartemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
593
-
artemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
881
-
dihydroartemisinic aldehyde
pH 8.5, 30°C
Artemisia annua
Other publictions for EC 1.14.13.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716656
Alejos-Gonzalez
Characterization of developmen ...
Artemisia annua
Planta
234
685-697
2011
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
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-
-
-
-
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718205
Zhang
The production of artemisinin ...
Artemisia annua
Plant Biotechnol. J.
9
445-454
2011
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706125
Olsson
Localization of enzymes of art ...
Artemisia annua
Phytochemistry
70
1123-1128
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
717445
Teoh
-
Molecular cloning of an aldehy ...
Artemisia annua
Botany
87
635-642
2009
-
-
1
-
-
-
-
2
-
-
1
-
-
2
-
-
-
-
-
1
-
-
13
1
-
-
-
2
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
13
1
-
-
-
2
1
-
-
-
-
-
-
-
2
2
717601
Teoh
Artemisia annua L. (Asteraceae ...
Artemisia annua
FEBS Lett.
580
1411-1416
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
4
-
-
-
-
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