BRENDA - Enzyme Database show
show all sequences of 1.14.13.158

The production of artemisinin precursors in tobacco

Zhang, Y.; Nowak, G.; Reed, D.W.; Covello, P.S.; Plant Biotechnol. J. 9, 445-454 (2011)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
heterologous expression of Artemisia annua amorphadiene synthase and CYP71AV1 in tobacco lead to the accumulation of amorphadiene and artemisinic alcohol, but not artemisinic acid. Additional expression of artemisinic aldehyde DELTA11(13) double-bond reductase DBR2 with or without aldehyde dehydrogenase 1 leads to the additional accumulation dihydroartemisinic alcohol. Results and suggest that amorphane sesquiterpenoid aldehydes are formed, but conditions in the transgenic tobacco cells favour reduction to alcohols rather than oxidation to acids
Artemisia annua
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Artemisia annua
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Application (protein specific)
Application
Commentary
Organism
synthesis
heterologous expression of Artemisia annua amorphadiene synthase and CYP71AV1 in tobacco lead to the accumulation of amorphadiene and artemisinic alcohol, but not artemisinic acid. Additional expression of artemisinic aldehyde DELTA11(13) double-bond reductase DBR2 with or without aldehyde dehydrogenase 1 leads to the additional accumulation dihydroartemisinic alcohol. Results and suggest that amorphane sesquiterpenoid aldehydes are formed, but conditions in the transgenic tobacco cells favour reduction to alcohols rather than oxidation to acids
Artemisia annua
Other publictions for EC 1.14.13.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716656
Alejos-Gonzalez
Characterization of developmen ...
Artemisia annua
Planta
234
685-697
2011
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718205
Zhang
The production of artemisinin ...
Artemisia annua
Plant Biotechnol. J.
9
445-454
2011
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706125
Olsson
Localization of enzymes of art ...
Artemisia annua
Phytochemistry
70
1123-1128
2009
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717445
Teoh
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Molecular cloning of an aldehy ...
Artemisia annua
Botany
87
635-642
2009
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717601
Teoh
Artemisia annua L. (Asteraceae ...
Artemisia annua
FEBS Lett.
580
1411-1416
2006
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