BRENDA - Enzyme Database show
show all sequences of 1.14.13.158

Glandular trichome-specific WRKY 1 promotes artemisinin biosynthesis in Artemisia annua

Chen, M.; Yan, T.; Shen, Q.; Lu, X.; Pan, Q.; Huang, Y.; Tang, Y.; Fu, X.; Liu, M.; Jiang, W.; Lv, Z.; Shi, P.; Ma, Y.; Hao, X.; Zhang, L.; Li, L.; Tang, K.; New Phytol. 214, 304-316 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP71AV1, quantitative real-time PCR enzyme expression analysis
Artemisia annua
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia annua
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + O2 + NADPH + H+
Artemisia annua
-
artemisinic alcohol + NADP+ + H2O
-
-
?
artemisinic alcohol + O2 + NADPH + H+
Artemisia annua
-
artemisinic aldehyde + NADP+ + 2 H2O
-
-
?
artemisinic aldehyde + O2 + NADPH + H+
Artemisia annua
-
artemisinate + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Artemisia annua
Q1PS23
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
secretory trichome
of stems, old and young leaves, and flower buds gathered 5-20 d after budding
Artemisia annua
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
745905
Artemisia annua
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + O2 + NADPH + H+
-
745905
Artemisia annua
artemisinic alcohol + NADP+ + H2O
-
-
-
?
artemisinic alcohol + O2 + NADPH + H+
-
745905
Artemisia annua
artemisinic aldehyde + NADP+ + 2 H2O
-
-
-
?
artemisinic aldehyde + O2 + NADPH + H+
-
745905
Artemisia annua
artemisinate + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Artemisia annua
NADPH
-
Artemisia annua
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP71AV1, quantitative real-time PCR enzyme expression analysis
Artemisia annua
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Artemisia annua
NADPH
-
Artemisia annua
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia annua
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + O2 + NADPH + H+
Artemisia annua
-
artemisinic alcohol + NADP+ + H2O
-
-
?
artemisinic alcohol + O2 + NADPH + H+
Artemisia annua
-
artemisinic aldehyde + NADP+ + 2 H2O
-
-
?
artemisinic aldehyde + O2 + NADPH + H+
Artemisia annua
-
artemisinate + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
secretory trichome
of stems, old and young leaves, and flower buds gathered 5-20 d after budding
Artemisia annua
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
745905
Artemisia annua
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + O2 + NADPH + H+
-
745905
Artemisia annua
artemisinic alcohol + NADP+ + H2O
-
-
-
?
artemisinic alcohol + O2 + NADPH + H+
-
745905
Artemisia annua
artemisinic aldehyde + NADP+ + 2 H2O
-
-
-
?
artemisinic aldehyde + O2 + NADPH + H+
-
745905
Artemisia annua
artemisinate + NADP+ + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Artemisia annua
transcription factor protein glandular trichome-specific WRKY 1, i.e. AaGSW1, promotes artemisinin biosynthesis in Artemisia annua by inducing CYP71AV1 expression. AaGSW1 positively regulates CYP71AV1 and AaORA expression by directly binding to the W-box motifs in their promoters. Overexpression of AaGSW1 in Artemisia annua (via Agrobacterium tumefaciens transformation method) significantly improves artemisinin and dihydroartemisinic acid contents. AaGSW1 can be directly regulated by AaMYC2 and AabZIP1, which are positive regulators of jasmonate- and abscisic acid-mediated artemisinin biosynthetic pathways, respectively. AaGSW1 binds to the W-box in the CYP71AV1 promoter in vitro and activates the transcription of CYP71AV1 in vivo. Global expression profile and phylogenetic analysis of WRKY transcription factors in Artemisia annua, overview
up
General Information
General Information
Commentary
Organism
physiological function
the positive regulator and transcription factor, glandular trichome-specific WRKY 1, is involved in the regulatory network of the artemisinin biosynthetic pathway, overview. Both AaMYC2 and AabZIP1 form a feed-forward loop with AaGSW1 to regulate CYP71AV1
Artemisia annua
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the positive regulator and transcription factor, glandular trichome-specific WRKY 1, is involved in the regulatory network of the artemisinin biosynthetic pathway, overview. Both AaMYC2 and AabZIP1 form a feed-forward loop with AaGSW1 to regulate CYP71AV1
Artemisia annua
Expression (protein specific)
Organism
Commentary
Expression
Artemisia annua
transcription factor protein glandular trichome-specific WRKY 1, i.e. AaGSW1, promotes artemisinin biosynthesis in Artemisia annua by inducing CYP71AV1 expression. AaGSW1 positively regulates CYP71AV1 and AaORA expression by directly binding to the W-box motifs in their promoters. Overexpression of AaGSW1 in Artemisia annua (via Agrobacterium tumefaciens transformation method) significantly improves artemisinin and dihydroartemisinic acid contents. AaGSW1 can be directly regulated by AaMYC2 and AabZIP1, which are positive regulators of jasmonate- and abscisic acid-mediated artemisinin biosynthetic pathways, respectively. AaGSW1 binds to the W-box in the CYP71AV1 promoter in vitro and activates the transcription of CYP71AV1 in vivo. Global expression profile and phylogenetic analysis of WRKY transcription factors in Artemisia annua, overview
up
Other publictions for EC 1.14.13.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745821
Chen
Multienzyme biosynthesis of d ...
Artemisia annua
Molecules
22
1422-1433
2017
-
1
1
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
3
-
-
-
-
1
1
3
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
745905
Chen
Glandular trichome-specific W ...
Artemisia annua
New Phytol.
214
304-316
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
746043
Muangphrom
Functional analysis of amorph ...
Artemisia absinthium, Artemisia afra, Artemisia annua, Artemisia kurramensis
Plant Cell Physiol.
57
1678-1688
2016
-
-
3
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
3
8
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
8
8
-
-
-
746069
Zheng
-
The influence of endophytic P ...
Artemisia annua
Plant Growth Regul.
80
93-102
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
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-
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-
1
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-
3
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
746309
Czechowski
Artemisia annua mutant impair ...
Artemisia annua
Proc. Natl. Acad. Sci. USA
113
15150-15155
2016
-
-
-
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
4
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
6
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
746035
Ji
Cloning and characterization ...
Artemisia annua
Plant Cell Physiol.
55
1592-1604
2014
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-