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show all sequences of 1.14.13.158

Cloning and characterization of AabHLH1, a bHLH transcription factor that positively regulates artemisinin biosynthesis in Artemisia annua

Ji, Y.; Xiao, J.; Shen, Y.; Ma, D.; Li, Z.; Pu, G.; Li, X.; Huang, L.; Liu, B.; Ye, H.; Wang, H.; Plant Cell Physiol. 55, 1592-1604 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP71AV1, semi-quantitative RT-PCR enzyme expression analysis, the expression level of CYP71AV1 significantly increases with the overexpression of AabHLH1
Artemisia annua
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia annua
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + O2 + NADPH + H+
Artemisia annua
-
artemisinic alcohol + NADP+ + H2O
-
-
?
artemisinic alcohol + O2 + NADPH + H+
Artemisia annua
-
artemisinic aldehyde + NADP+ + 2 H2O
-
-
?
artemisinic aldehyde + O2 + NADPH + H+
Artemisia annua
-
artemisinate + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Artemisia annua
Q1PS23
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
glandular secretory trichome
of leaves
Artemisia annua
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
746035
Artemisia annua
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + O2 + NADPH + H+
-
746035
Artemisia annua
artemisinic alcohol + NADP+ + H2O
-
-
-
?
artemisinic alcohol + O2 + NADPH + H+
-
746035
Artemisia annua
artemisinic aldehyde + NADP+ + 2 H2O
-
-
-
?
artemisinic aldehyde + O2 + NADPH + H+
-
746035
Artemisia annua
artemisinate + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Artemisia annua
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP71AV1, semi-quantitative RT-PCR enzyme expression analysis, the expression level of CYP71AV1 significantly increases with the overexpression of AabHLH1
Artemisia annua
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Artemisia annua
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia annua
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + O2 + NADPH + H+
Artemisia annua
-
artemisinic alcohol + NADP+ + H2O
-
-
?
artemisinic alcohol + O2 + NADPH + H+
Artemisia annua
-
artemisinic aldehyde + NADP+ + 2 H2O
-
-
?
artemisinic aldehyde + O2 + NADPH + H+
Artemisia annua
-
artemisinate + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
glandular secretory trichome
of leaves
Artemisia annua
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
746035
Artemisia annua
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + O2 + NADPH + H+
-
746035
Artemisia annua
artemisinic alcohol + NADP+ + H2O
-
-
-
?
artemisinic alcohol + O2 + NADPH + H+
-
746035
Artemisia annua
artemisinic aldehyde + NADP+ + 2 H2O
-
-
-
?
artemisinic aldehyde + O2 + NADPH + H+
-
746035
Artemisia annua
artemisinate + NADP+ + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Artemisia annua
the expression level of CYP71AV1 significantly increases with the overexpression of transcription factor AabHLH1. The promoters of ADS and CYP71AV1 contain E-box elements, which are putative binding sites for basic helix-loop-helix (bHLH) transcription factors. The E-box is important for the CYP71AV1 promoter activity. AabHLH1 can positively regulate the biosynthesis of artemisinin. AabHLH1 protein is capable of binding to the E-box cis-elements, present in both ADS and CYP71AV1 promoters, and possesses transactivation activity in yeast. Transient expression of AabHLH1 in Artemisia annua leaves increases transcript levels of the genes involved in artemisinin biosynthesis, such as ADS, CYP71AV1 and HMGR
up
General Information
General Information
Commentary
Organism
malfunction
abolished activation of AabHLH1 by mutated E-boxes of the CYP71AV1 promoter
Artemisia annua
metabolism
amorpha-4,11-diene synthase (ADS) and Cyt P450 monooxygenase CYP71AV1 in Artemisia annua are two key enzymes involved in the biosynthesis of artemisinin. The promoters of ADS and CYP71AV1 contain E-box elements, which are putative binding sites for basic helix-loop-helix (bHLH) transcription factors. The E-box is important for the CYP71AV1 promoter activity. AabHLH1 can positively regulate the biosynthesis of artemisinin. AabHLH1 protein is capable of binding to the E-box cis-elements, present in both ADS and CYP71AV1 promoters, and possesses transactivation activity in yeast
Artemisia annua
General Information (protein specific)
General Information
Commentary
Organism
malfunction
abolished activation of AabHLH1 by mutated E-boxes of the CYP71AV1 promoter
Artemisia annua
metabolism
amorpha-4,11-diene synthase (ADS) and Cyt P450 monooxygenase CYP71AV1 in Artemisia annua are two key enzymes involved in the biosynthesis of artemisinin. The promoters of ADS and CYP71AV1 contain E-box elements, which are putative binding sites for basic helix-loop-helix (bHLH) transcription factors. The E-box is important for the CYP71AV1 promoter activity. AabHLH1 can positively regulate the biosynthesis of artemisinin. AabHLH1 protein is capable of binding to the E-box cis-elements, present in both ADS and CYP71AV1 promoters, and possesses transactivation activity in yeast
Artemisia annua
Expression (protein specific)
Organism
Commentary
Expression
Artemisia annua
the expression level of CYP71AV1 significantly increases with the overexpression of transcription factor AabHLH1. The promoters of ADS and CYP71AV1 contain E-box elements, which are putative binding sites for basic helix-loop-helix (bHLH) transcription factors. The E-box is important for the CYP71AV1 promoter activity. AabHLH1 can positively regulate the biosynthesis of artemisinin. AabHLH1 protein is capable of binding to the E-box cis-elements, present in both ADS and CYP71AV1 promoters, and possesses transactivation activity in yeast. Transient expression of AabHLH1 in Artemisia annua leaves increases transcript levels of the genes involved in artemisinin biosynthesis, such as ADS, CYP71AV1 and HMGR
up
Other publictions for EC 1.14.13.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745821
Chen
Multienzyme biosynthesis of d ...
Artemisia annua
Molecules
22
1422-1433
2017
-
1
1
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1
-
-
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4
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1
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4
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1
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3
-
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1
1
3
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1
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-
-
4
-
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-
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-
-
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4
-
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-
-
-
1
-
-
-
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2
2
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-
-
745905
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Glandular trichome-specific W ...
Artemisia annua
New Phytol.
214
304-316
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
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1
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4
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2
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1
2
-
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4
-
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1
-
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4
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-
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1
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1
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746043
Muangphrom
Functional analysis of amorph ...
Artemisia absinthium, Artemisia afra, Artemisia annua, Artemisia kurramensis
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2016
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3
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4
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8
-
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-
-
3
8
-
-
-
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-
-
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4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
8
8
-
-
-
746069
Zheng
-
The influence of endophytic P ...
Artemisia annua
Plant Growth Regul.
80
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2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
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1
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3
-
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1
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-
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1
-
-
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1
-
-
1
-
-
746309
Czechowski
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Artemisia annua
Proc. Natl. Acad. Sci. USA
113
15150-15155
2016
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-
-
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1
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-
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-
-
-
4
-
1
-
-
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-
-
4
-
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6
-
-
-
-
-
-
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-
2
-
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-
2
-
1
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4
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4
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6
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3
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746035
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Cloning and characterization ...
Artemisia annua
Plant Cell Physiol.
55
1592-1604
2014
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1
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4
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1
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1
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4
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1
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1
1
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4
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1
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4
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1
2
2
1
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