BRENDA - Enzyme Database show
show all sequences of 1.14.13.158

Functional analysis of amorpha-4,11-diene synthase (ADS) homologs from non-artemisinin-producing artemisia species the discovery of novel koidzumiol and (+)-alpha-bisabolol synthases

Muangphrom, P.; Seki, H.; Suzuki, M.; Komori, A.; Nishiwaki, M.; Mikawa, R.; Fukushima, E.O.; Muranaka, T.; Plant Cell Physiol. 57, 1678-1688 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
Artemisia absinthium
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
Artemisia afra
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
Artemisia annua
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia annua
-
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia afra
-
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia absinthium
-
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia kurramensis
-
artemisinate + 3 NADP+ + 4 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Artemisia kurramensis
-
-
-
Artemisia absinthium
K7ZPJ9
-
-
Artemisia afra
K7ZR99
-
-
Artemisia annua
Q1PS23
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Artemisia absinthium
-
leaf
-
Artemisia afra
-
leaf
-
Artemisia annua
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
-
746043
Artemisia annua
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
-
746043
Artemisia afra
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
-
746043
Artemisia absinthium
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
-
746043
Artemisia kurramensis
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Artemisia absinthium
cytochrome P450
-
Artemisia afra
cytochrome P450
-
Artemisia kurramensis
cytochrome P450
-
Artemisia annua
NADPH
-
Artemisia absinthium
NADPH
-
Artemisia afra
NADPH
-
Artemisia kurramensis
NADPH
-
Artemisia annua
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
Artemisia absinthium
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
Artemisia afra
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
Artemisia annua
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Artemisia absinthium
cytochrome P450
-
Artemisia afra
cytochrome P450
-
Artemisia kurramensis
cytochrome P450
-
Artemisia annua
NADPH
-
Artemisia absinthium
NADPH
-
Artemisia afra
NADPH
-
Artemisia kurramensis
NADPH
-
Artemisia annua
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia annua
-
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia afra
-
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia absinthium
-
artemisinate + 3 NADP+ + 4 H2O
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
Artemisia kurramensis
-
artemisinate + 3 NADP+ + 4 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Artemisia absinthium
-
leaf
-
Artemisia afra
-
leaf
-
Artemisia annua
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
-
746043
Artemisia annua
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
-
746043
Artemisia afra
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
-
746043
Artemisia absinthium
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
amorpha-4,11-diene + 3 O2 + 3 NADPH + 3 H+
-
746043
Artemisia kurramensis
artemisinate + 3 NADP+ + 4 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the artemisinin biosynthesis
Artemisia absinthium
metabolism
the enzyme is involved in the artemisinin biosynthesis
Artemisia afra
metabolism
the enzyme is involved in the artemisinin biosynthesis
Artemisia annua
metabolism
the enzyme is involved in the artemisinin biosynthesis
Artemisia kurramensis
physiological function
amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde
Artemisia absinthium
physiological function
amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde
Artemisia afra
physiological function
amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde
Artemisia annua
physiological function
amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde
Artemisia kurramensis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the artemisinin biosynthesis
Artemisia absinthium
metabolism
the enzyme is involved in the artemisinin biosynthesis
Artemisia afra
metabolism
the enzyme is involved in the artemisinin biosynthesis
Artemisia annua
metabolism
the enzyme is involved in the artemisinin biosynthesis
Artemisia kurramensis
physiological function
amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde
Artemisia absinthium
physiological function
amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde
Artemisia afra
physiological function
amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde
Artemisia annua
physiological function
amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde
Artemisia kurramensis
Other publictions for EC 1.14.13.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745821
Chen
Multienzyme biosynthesis of d ...
Artemisia annua
Molecules
22
1422-1433
2017
-
1
1
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
3
-
-
-
-
1
1
3
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
745905
Chen
Glandular trichome-specific W ...
Artemisia annua
New Phytol.
214
304-316
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
746043
Muangphrom
Functional analysis of amorph ...
Artemisia absinthium, Artemisia afra, Artemisia annua, Artemisia kurramensis
Plant Cell Physiol.
57
1678-1688
2016
-
-
3
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
3
8
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
8
8
-
-
-
746069
Zheng
-
The influence of endophytic P ...
Artemisia annua
Plant Growth Regul.
80
93-102
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
746309
Czechowski
Artemisia annua mutant impair ...
Artemisia annua
Proc. Natl. Acad. Sci. USA
113
15150-15155
2016
-
-
-
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
4
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
6
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
746035
Ji
Cloning and characterization ...
Artemisia annua
Plant Cell Physiol.
55
1592-1604
2014
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-