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Literature summary for 1.14.13.161 extracted from

  • Funk, C.; Croteau, R.
    Induction and characterization of a cytochrome P-450-dependent camphor hydroxylase in tissue cultures of common sage (Salvia officinalis) (1993), Plant Physiol., 101, 1231-1237.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
FAD inclusion of both FAD and FMN in the incubation mixture enhances hydroxylation activity 2.4fold Salvia officinalis
FMN inclusion of both FAD and FMN in the incubation mixture enhances hydroxylation activity 2.4fold Salvia officinalis

Inhibitors

Inhibitors Comment Organism Structure
clotrimazole 84% inhibition at 0.05 mM Salvia officinalis
CO 73% inhibition at CO:O2 ratio of 9:1, activity is partially restored by blue light Salvia officinalis
cytochrome c 95% inhibition at 0.05 mM Salvia officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
(+)-Camphor pH 7.0, 32Ā°C Salvia officinalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane microsomal membrane Salvia officinalis 16020
-
microsome microsomal membrane Salvia officinalis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 upon treatment of sage suspension cultures with 30 mM MnCI2, camphor-6-hydroxylase activity is induced up to 7fold Salvia officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
x * 58000, SDS-PAGE Salvia officinalis

Organism

Organism UniProt Comment Textmining
Salvia officinalis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Salvia officinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + NADPH + H+ + O2
-
Salvia officinalis (+)-6-exo-hydroxycamphor + NADP+ + H2O
-
?
additional information the microsomal camphor-6-exo-hydroxylase system requires molecular oxygen and a reduced pyridine nucleotide Salvia officinalis ?
-
?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Salvia officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Salvia officinalis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 more than 60% of maximum activity within Salvia officinalis

Cofactor

Cofactor Comment Organism Structure
NADH 21% of the rate with NADPH Salvia officinalis
NADPH
-
Salvia officinalis