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Literature summary for extracted from

  • Wei, M.; Zhang, J.J.; Liu, H.; Zhou, N.Y.
    para-Nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in Pseudomonas sp. strain WBC-3 (2010), Biodegradation, 21, 915-921.
    View publication on PubMed


Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp.


Organism UniProt Comment Textmining
Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrocatechol + NADPH + H+ + O2 monooxygenation, the enzyme completely degrades 0.1 mM 4-nitrocatechol in 80 min Pseudomonas sp. 1,2,4-trihydroxybenzene + nitrite + NADP+ + H2O


Synonyms Comment Organism
para-nitrophenol 4-monooxygenase
Pseudomonas sp.
PNP 4-monooxygenase
Pseudomonas sp.
Pseudomonas sp.


Cofactor Comment Organism Structure
Pseudomonas sp.


Organism Comment Expression
Pseudomonas sp. enzyme expression can be induced by 4-nitrophenol but not 4-nitrocatechol up