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Literature summary for 1.14.13.170 extracted from

  • Seo, M.J.; Zhu, D.; Endo, S.; Ikeda, H.; Cane, D.E.
    Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone (2011), Biochemistry, 50, 1739-1754.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces exfoliatus
expressed in Escherichia coli BL21(DE3) cells Streptomyces arenae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66915
-
 x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry Streptomyces arenae
67480
-
 x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry Streptomyces exfoliatus

Organism

Organism UniProt Comment Textmining
Streptomyces arenae
-
-
-
Streptomyces arenae TU46
-
-
-
Streptomyces exfoliatus
-
-
-
Streptomyces exfoliatus UC5319
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptomyces exfoliatus
Ni-NTA column chromatography Streptomyces arenae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
 Streptomyces exfoliatus pentalenolactone D + NADP+ + H2O
-
 ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
 Streptomyces arenae pentalenolactone D + NADP+ + H2O
-
 ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
 Streptomyces arenae TU46 pentalenolactone D + NADP+ + H2O
-
 ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
 Streptomyces exfoliatus UC5319 pentalenolactone D + NADP+ + H2O
-
 ir
1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2
-
 Streptomyces arenae TUE469 pentalenolactone D + NADP+ + H2O
-
 ir

Subunits

Subunits Comment Organism
? x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry Streptomyces arenae
? x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry Streptomyces exfoliatus

Synonyms

Synonyms Comment Organism
Baeyer-Villiger monooxygenase
-
 Streptomyces exfoliatus
Baeyer-Villiger monooxygenase
-
 Streptomyces arenae
penE
-
 Streptomyces exfoliatus
pntE
-
 Streptomyces arenae

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains one bound FAD molecule Streptomyces exfoliatus
FAD the enzyme contains one bound FAD molecule Streptomyces arenae