Application | Comment | Organism |
---|---|---|
synthesis | biosynthesis of carminic acid from glucose in engineered Escherichia coli. Expression of an optimized type II polyketide synthase machinery from Photorhabdus luminescens enables a high-level production of flavokermesic acid upon coexpression of the cyclases ZhuI and ZhuJ from Streptomyces sp. R1128. Mutants of aklavinone 12-hydroxylase (DnrF) from Streptomyces peucetius and C-glucosyltransferase (GtCGT) from Gentiana triflora with 2-5fold enhanced conversion efficiencies can perform hydroxylation and C-glucosylation of flavokermesic acid, respectively, resulting in 0.63 mg/l of carminic acid production from glucose | Streptomyces peucetius |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure using RdmE from Streptomyces purpurascens, PDB ID 3IHG | Streptomyces peucetius |
Protein Variants | Comment | Organism |
---|---|---|
M56A | 2fold increase in synthesis of kermesic acid | Streptomyces peucetius |
P217K | 2.2fold increase in synthesis of kermesic acid | Streptomyces peucetius |
P217K/M56A | significant decrease in synthesis of kermesic acid | Streptomyces peucetius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0433 | - |
flavokermesic acid | mutant P217K, pH not specified in the publication, temperature not specified in the publication | Streptomyces peucetius | |
0.0568 | - |
flavokermesic acid | wild-type, pH not specified in the publication, temperature not specified in the publication | Streptomyces peucetius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces peucetius | P32009 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C-glucosyl flavokermesic acid + NADPH + H+ + O2 | - |
Streptomyces peucetius | carminic acid + NADP+ + H2O | - |
? | |
flavokermesic acid + NADPH + H+ + O2 | - |
Streptomyces peucetius | kermesic acid + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aklavinone 12-hydroxylase | - |
Streptomyces peucetius |
DnrF | - |
Streptomyces peucetius |