Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.181 extracted from

  • Rimal, H.; Lee, S.; Lee, J.; Oh, T.
    Understanding of real alternative redox partner of Streptomyces peucetius DoxA Prediction and validation using in silico and in vitro analyses (2015), Arch. Biochem. Biophys., 585, 64-74 .
    View publication on PubMed
Search for more literature for 1.14.13.181

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of His-tagged enzyme DoxA along with its His-tagged redox partners from Streptomyces peucetius FDX1, FDR2, and FDX3, and the putidaredoxin and putidaredoxin reductase from Pseudomonas putida, that are essential equivalents of the class I type of bacterial electron-transport system, in Escherichia coli strain BL21(DE3) harboring the GroES/EL-expressing plasmid pGro7 Streptomyces peucetius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
daunorubicin + NADPH + H+ + O2 Streptomyces peucetius
-
 doxorubicin + NADP+ + H2O
-
 ?
daunorubicin + NADPH + H+ + O2 Streptomyces peucetius ATCC 27952
-
 doxorubicin + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces peucetius
-
-
-
Streptomyces peucetius ATCC 27952
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme DoxA from Escherichia coli, copurification with His-tagged FDX1, FDR2, FDX3, and putidaredoxin and putidaredoxin reductase from Pseudomonas putida from Escherichia coli by cobalt affinity chromatography and ultrafiltration Streptomyces peucetius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
daunorubicin + NADPH + H+ + O2
-
 Streptomyces peucetius doxorubicin + NADP+ + H2O
-
 ?
daunorubicin + NADPH + H+ + O2
-
 Streptomyces peucetius ATCC 27952 doxorubicin + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DoxA
-
 Streptomyces peucetius

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Streptomyces peucetius
NADPH
-
 Streptomyces peucetius

General Information

General Information Comment Organism
additional information protein-protein interaction and docking analysis of recombinant enzymes, structure homology modeling, DoxA active site structure, overview. Enzyme DoxA interacts with ist redox partners FDX1, FDR2, and FDX3 Streptomyces peucetius
physiological function the cytochrome P450 monoxygenase DoxA is responsible for the hydroxylation of daunorubicin into doxorubicin Streptomyces peucetius