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Literature summary for 1.14.13.195 extracted from

  • Ambrosi, C.; Leoni, L.; Putignani, L.; Orsi, N.; Visca, P.
    Pseudobactin biogenesis, in the plant growth-promoting rhizobacterium Pseudomonas strain B10: Identification and functional analysis of the L-ornithine N5-oxygenase (psbA) gene (2000), J. Bacteriol., 182, 6233-6238.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene psbA, DNA and amino acid sequence determination and analysis, monocistronic gene Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information a PsbA-defective mutant B10CA1 is fully complemented by addition of L-ornithine N5-oxide, the mutant strain shows impaired synthesis of fluorescent pigment and hydroxamate nitrogen in iron-poor medium, as well as defective biosynthesis of both forms of pseudobactin and salicylate-based siderophores, Fe3+ transfer is impaired, phenotype, overview Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the psbA expression is negatively controlled by iron Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine + NADPH + H+ + O2 Pseudomonas sp.
-
 N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2 Pseudomonas sp. B10
-
 N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q9F8X0 gene psbA
-
Pseudomonas sp. B10 Q9F8X0 gene psbA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + NADPH + H+ + O2
-
 Pseudomonas sp. N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2 the enzyme contains the FATGY signature in the putative substrate binding pocket Pseudomonas sp. N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2
-
 Pseudomonas sp. B10 N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
L-ornithine + NADPH + H+ + O2 the enzyme contains the FATGY signature in the putative substrate binding pocket Pseudomonas sp. B10 N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
L-Orn N5-oxygenase
-
 Pseudomonas sp.
PsbA
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas sp.
NADPH
-
 Pseudomonas sp.

Expression

Organism Comment Expression
Pseudomonas sp. the psbA expression is negatively controlled by iron down