Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, mutant enzyme S212A | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
S212A | the turnover of the substrate 2,4-dihydroxybenzoate is 1.5-fold faster than the rate observed with the wild-type | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P20586 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dihydroxybenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | protocatechuate + NADP+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | movement of the flavin occurs before reduction | Pseudomonas aeruginosa |