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Literature summary for 1.14.13.2 extracted from

  • Moran, G.R.; Entsch, B.; Palfey, B.A.; Ballou, D.P.
    Mechanistic insights into p-hydroxybenzoate hydroxylase from studies of the mutant Ser212Ala (1999), Biochemistry, 38, 6292-6299.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, mutant enzyme S212A Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
S212A the turnover of the substrate 2,4-dihydroxybenzoate is 1.5-fold faster than the rate observed with the wild-type Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P20586
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dihydroxybenzoate + NADPH + O2
-
Pseudomonas aeruginosa 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas aeruginosa protocatechuate + NADP+ + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
FAD movement of the flavin occurs before reduction Pseudomonas aeruginosa