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Literature summary for 1.14.13.2 extracted from

  • Fernandez, J.; Dimarco, A.A.; Ornston, L.N.; Harayama, S.
    Purification and characterization of Acinetobacter calcoaceticus 4-hydroxybenzoate 3-hydroxylase after its overexpression in Escherichia coli (1995), J. Biochem., 117, 1261-1266.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Acinetobacter calcoaceticus

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dihydroxybenzoate competitive with 4-hydroxybenzoate Acinetobacter calcoaceticus
3,4-dihydroxybenzoate competitive with 4-hydroxybenzoate Acinetobacter calcoaceticus
3-Chloro-4-hydroxybenzoate mixed-type Acinetobacter calcoaceticus
4-Aminobenzoate competitive Acinetobacter calcoaceticus
4-hydroxybenzoate above 1 mM Acinetobacter calcoaceticus
benzoate competitive with 4-hydroxybenzoate Acinetobacter calcoaceticus
Cl- non-competitive with NADPH Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
4-hydroxybenzoate
-
Acinetobacter calcoaceticus
0.18
-
3-Chloro-4-hydroxybenzoate
-
Acinetobacter calcoaceticus
0.25
-
2,4-Dihydroxybenzoate
-
Acinetobacter calcoaceticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
gel filtration Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38
-
-
Acinetobacter calcoaceticus

Storage Stability

Storage Stability Organism
4°C, 24 h, 40% loss of activity without stabilizer, 10% loss of activity in presence of 4-hydroxybenzoate, FAD and EDTA Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dihydroxybenzoate + NADPH + O2 3.1% of the activity with 4-hydroxybenzoate Acinetobacter calcoaceticus 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
?
3-Chloro-4-hydroxybenzoate + NADPH + O2 6.5% of the activity with 4-hydroxybenzoate Acinetobacter calcoaceticus ?
-
?
4-hydroxybenzoate + NADPH + O2
-
Acinetobacter calcoaceticus protocatechuate + NADP+ + H2O
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
24 h, 40% loss of activity without stabilizer, 10% loss of activity in presence of 4-hydroxybenzoate, FAD and EDTA Acinetobacter calcoaceticus
50
-
10 min, 60% loss of activity, in presence of 0.1 mM 4-hydroxybenzoate the enzyme retains 80% of its activity Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
NADPH no activity with NADH Acinetobacter calcoaceticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
4-Aminobenzoate
-
Acinetobacter calcoaceticus
0.31
-
2,4-Dihydroxybenzoate
-
Acinetobacter calcoaceticus
0.53
-
benzoate
-
Acinetobacter calcoaceticus
0.55
-
3,4-dihydroxybenzoate
-
Acinetobacter calcoaceticus