Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Acinetobacter calcoaceticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Dihydroxybenzoate | competitive with 4-hydroxybenzoate | Acinetobacter calcoaceticus | |
3,4-dihydroxybenzoate | competitive with 4-hydroxybenzoate | Acinetobacter calcoaceticus | |
3-Chloro-4-hydroxybenzoate | mixed-type | Acinetobacter calcoaceticus | |
4-Aminobenzoate | competitive | Acinetobacter calcoaceticus | |
4-hydroxybenzoate | above 1 mM | Acinetobacter calcoaceticus | |
benzoate | competitive with 4-hydroxybenzoate | Acinetobacter calcoaceticus | |
Cl- | non-competitive with NADPH | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
4-hydroxybenzoate | - |
Acinetobacter calcoaceticus | |
0.18 | - |
3-Chloro-4-hydroxybenzoate | - |
Acinetobacter calcoaceticus | |
0.25 | - |
2,4-Dihydroxybenzoate | - |
Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
gel filtration | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Acinetobacter calcoaceticus |
Storage Stability | Organism |
---|---|
4°C, 24 h, 40% loss of activity without stabilizer, 10% loss of activity in presence of 4-hydroxybenzoate, FAD and EDTA | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dihydroxybenzoate + NADPH + O2 | 3.1% of the activity with 4-hydroxybenzoate | Acinetobacter calcoaceticus | 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O | - |
? | |
3-Chloro-4-hydroxybenzoate + NADPH + O2 | 6.5% of the activity with 4-hydroxybenzoate | Acinetobacter calcoaceticus | ? | - |
? | |
4-hydroxybenzoate + NADPH + O2 | - |
Acinetobacter calcoaceticus | protocatechuate + NADP+ + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
24 h, 40% loss of activity without stabilizer, 10% loss of activity in presence of 4-hydroxybenzoate, FAD and EDTA | Acinetobacter calcoaceticus |
50 | - |
10 min, 60% loss of activity, in presence of 0.1 mM 4-hydroxybenzoate the enzyme retains 80% of its activity | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | no activity with NADH | Acinetobacter calcoaceticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
4-Aminobenzoate | - |
Acinetobacter calcoaceticus | |
0.31 | - |
2,4-Dihydroxybenzoate | - |
Acinetobacter calcoaceticus | |
0.53 | - |
benzoate | - |
Acinetobacter calcoaceticus | |
0.55 | - |
3,4-dihydroxybenzoate | - |
Acinetobacter calcoaceticus |