Crystallization (Comment) | Organism |
---|---|
crystallization of mutant enzymes H162R and R269T by hanging drop vapour diffusion method | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
H162D | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
H162K | less efficient than wild-type enzyme due to a clear increase in the apparent Km-value for NADPH | Pseudomonas fluorescens |
H162N | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
H162R | rather efficient enzyme with similar catalytic properties as wild-type enzyme | Pseudomonas fluorescens |
H162S | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
H162T | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
H162Y | rather efficient enzyme with similar catalytic properties as wild-type enzyme | Pseudomonas fluorescens |
R269D | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
R269K | rather efficient enzyme with similar catalytic properties as wild-type enzyme | Pseudomonas fluorescens |
R269N | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
R269S | less efficient than wild-type enzyme due to a clear increase in the apparent Km-value for NADPH | Pseudomonas fluorescens |
R269T | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
R269Y | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | inhibition of wild-type enzyme, no inhibition of mutant enzyme H162R | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for NADPH above 0.5 mM: mutant enzymes R269D, R269T, R269N, R269Y, H162D, H162T, H162S, H162N | Pseudomonas fluorescens | |
0.02 | - |
4-hydroxybenzoate | wild-type enzyme | Pseudomonas fluorescens | |
0.03 | - |
NADPH | wild-type enzyme | Pseudomonas fluorescens | |
0.03 | - |
4-hydroxybenzoate | mutant enzyme H162R, H162Y, H162K, R269K, R269Y, R269N, R269S and R269T | Pseudomonas fluorescens | |
0.04 | - |
4-hydroxybenzoate | mutant enzyme H162N, H162S, H162T and H162D | Pseudomonas fluorescens | |
0.05 | - |
NADPH | mutant enzyme H162R | Pseudomonas fluorescens | |
0.06 | - |
4-hydroxybenzoate | mutant enzyme R269D | Pseudomonas fluorescens | |
0.07 | - |
NADPH | mutant enzyme H162Y and R269K | Pseudomonas fluorescens | |
0.2 | - |
NADPH | mutant enzyme H162K | Pseudomonas fluorescens | |
0.32 | - |
NADPH | mutant enzyme R269S | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas fluorescens | protocatechuate + NADP+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas fluorescens |