Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
Y385F | the mutant displays higher activity toward 3,4-dihydroxybenzoic acid than the wild type enzyme | Pseudomonas aeruginosa |
Y385F/T294A | the mutant displays much higher activity toward 3,4-dihydroxybenzoic acid than the wild type enzyme | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01955 | - |
4-hydroxybenzoate | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.03467 | - |
4-hydroxybenzoate | wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.04838 | - |
4-hydroxybenzoate | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.15702 | - |
3,4-dihydroxybenzoic acid | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.22802 | - |
3,4-dihydroxybenzoic acid | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + H+ + O2 | Pseudomonas aeruginosa | - |
protocatechuate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.26 | - |
mutant enzyme Y385F, with 4-hydroxybenzoate as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
0.45 | - |
mutant enzyme Y385F, with 3,4-dihydroxybenzoic acid as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
1.04 | - |
mutant enzyme Y385F/T294A, with 4-hydroxybenzoate as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
1.86 | - |
mutant enzyme Y385F/T294A, with 3,4-dihydroxybenzoic acid as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
16.85 | - |
wild type enzyme, with 4-hydroxybenzoate as substrate, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoic acid + NADPH + H+ + O2 | weak activity | Pseudomonas aeruginosa | gallic acid + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + H+ + O2 | - |
Pseudomonas aeruginosa | protocatechuate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
p-hydroxybenzoate hydroxylase | - |
Pseudomonas aeruginosa |
PobA | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
4-hydroxybenzoate | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.39 | - |
3,4-dihydroxybenzoic acid | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
0.9 | - |
4-hydroxybenzoate | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
1.69 | - |
3,4-dihydroxybenzoic acid | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
14.12 | - |
4-hydroxybenzoate | wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas aeruginosa | |
NADPH | - |
Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
3,4-dihydroxybenzoic acid | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
10 | - |
4-hydroxybenzoate | mutant Y385F, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
12 | - |
3,4-dihydroxybenzoic acid | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
20 | - |
4-hydroxybenzoate | mutant Y385F/T294A, at pH 8.0 and 30°C | Pseudomonas aeruginosa | |
410 | - |
4-hydroxybenzoate | wild type enzyme, at pH 8.0 and 30°C | Pseudomonas aeruginosa |