General Stability | Organism |
---|---|
2,4-dichlorophenol, 0.1 mM, stabilizes during purification | Acinetobacter sp. |
dithiothreitol, 1 mM, stabilizes during purification | Acinetobacter sp. |
EDTA, 0.1 mM, stabilizes during purification | Acinetobacter sp. |
FAD, 0.01 mM, stabilizes during purification | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Dichlorophenol | above 1 mM | Acinetobacter sp. | |
AgNO3 | - |
Acinetobacter sp. | |
CuSO4 | - |
Acinetobacter sp. | |
diethyldithiocarbamate | - |
Acinetobacter sp. | |
dithiothreitol | slight | Acinetobacter sp. | |
FeCl3 | - |
Acinetobacter sp. | |
FeSO4 | - |
Acinetobacter sp. | |
Hg2+ | HgCl2 | Acinetobacter sp. | |
KCN | - |
Acinetobacter sp. | |
p-chloromercuribenzoate | - |
Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
2,4-Dichlorophenol | cosubstrate NADPH | Acinetobacter sp. | |
0.057 | - |
O2 | - |
Acinetobacter sp. | |
0.083 | - |
NADPH | - |
Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
alpha4, 4 * 63000, SDS-PAGE | Acinetobacter sp. |
240000 | - |
gel filtration | Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + NADPH + O2 | Acinetobacter sp. | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.46 | - |
- |
Acinetobacter sp. |
Storage Stability | Organism |
---|---|
-20°C, 3 months stable | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + NADPH + O2 | - |
Acinetobacter sp. | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2-chlorophenol + NADPH + O2 | incompletely metabolized | Acinetobacter sp. | ? | - |
? | |
2-cresol + NADPH + O2 | incompletely metabolized | Acinetobacter sp. | ? | - |
? | |
4-bromophenol + NADPH + O2 | incompletely metabolized | Acinetobacter sp. | ? | - |
? | |
4-chloro-2-methylphenol + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
4-chlorophenol + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
additional information | pseudosubstrates evoke oxidation of NAD(P)H and oxygen consumption without themselves undergoing hydroxylation, the product is hydrogen peroxide: 2,5-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2, 4,5-trichlorophenol | Acinetobacter sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 63000, SDS-PAGE | Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
29 | - |
- |
Acinetobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.83 | - |
2,4-Dichlorophenol | - |
Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Acinetobacter sp. | |
FAD | FAD cannot be replaced by riboflavin or FMN | Acinetobacter sp. | |
FAD | 1 mol of enzyme contains: 1.9 mol FAD | Acinetobacter sp. | |
NADH | 5% of the activity with NADPH | Acinetobacter sp. | |
NADPH | - |
Acinetobacter sp. |