Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli and Saccharomyces cerevisiae | Acinetobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | His-tagged recombinant enzyme expressed in Escherichia coli is, despite the tag, similar to the wild-type enzyme, whereas the recombinant His-tagged enzyme expressed in yeast is posttranslationally modified, Escherichia coli is the preferred expression system | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60800 | - |
wild-type, electrospray mass spectrometry | Acinetobacter sp. |
61620 | - |
recombinant His-tagged enzyme expressed in E. coli | Acinetobacter sp. |
61670 | - |
recombinant His-tagged enzyme expressed in yeast | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | Q9R2F5 | NCIB 9871 | - |
Purification (Comment) | Organism |
---|---|
native enzyme and recombinant His-tagged enzyme from Escherichia coli and Saccharomyces cerevisiae | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
purified recombinant His-tagged enzyme expressed in yeast | Acinetobacter sp. |
15 | - |
purified native enzyme | Acinetobacter sp. |
19.9 | - |
purified recombinant His-tagged enzyme expressed in E. coli | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? |
Synonyms | Comment | Organism |
---|---|---|
CMO | - |
Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Acinetobacter sp. | |
NADPH | - |
Acinetobacter sp. |