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Literature summary for 1.14.13.22 extracted from

  • Cheesman, M.J.; Kneller, M.B.; Kelly, E.J.; Thompson, S.J.; Yeung, C.K.; Eaton, D.L.; Rettie, A.E.
    purification and characterization of hexahistidine-tagged cyclohexanone monooxygenase expressed in Saccharomyces cerevisiae and Escherichia coli (2001), Protein Expr. Purif., 21, 81-86.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli and Saccharomyces cerevisiae Acinetobacter sp.

Protein Variants

Protein Variants Comment Organism
additional information His-tagged recombinant enzyme expressed in Escherichia coli is, despite the tag, similar to the wild-type enzyme, whereas the recombinant His-tagged enzyme expressed in yeast is posttranslationally modified, Escherichia coli is the preferred expression system Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60800
-
wild-type, electrospray mass spectrometry Acinetobacter sp.
61620
-
recombinant His-tagged enzyme expressed in E. coli Acinetobacter sp.
61670
-
recombinant His-tagged enzyme expressed in yeast Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. Q9R2F5 NCIB 9871
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme and recombinant His-tagged enzyme from Escherichia coli and Saccharomyces cerevisiae Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
purified recombinant His-tagged enzyme expressed in yeast Acinetobacter sp.
15
-
purified native enzyme Acinetobacter sp.
19.9
-
purified recombinant His-tagged enzyme expressed in E. coli Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexanone + NADPH + O2
-
Acinetobacter sp. 6-hexanolide + NADP+ + H2O product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane ?

Synonyms

Synonyms Comment Organism
CMO
-
Acinetobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
Acinetobacter sp.
NADPH
-
Acinetobacter sp.