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Literature summary for 1.14.13.22 extracted from

  • Brzostowicz, P.C.; Walters, D.M.; Thomas, S.M.; Nagarajan, V.; Rouviere, P.E.
    mRNA differential display in a microbial enrichment culture: simultaneous identification of three cyclohexanone monooxygenases from three species (2003), Appl. Environ. Microbiol., 69, 334-342.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli Rhodococcus pyridinivorans
expressed as His-tag fusion protein in Escherichia coli Arthrobacter sp.
expressed as His-tag fusion protein in Escherichia coli Rhodococcus ruber

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexanone + NADPH + O2 Rhodococcus ruber initial step of the degradation of cyclohexanone to adipic acid epsilon-caprolactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2 Rhodococcus pyridinivorans
-
hexano-6-lactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2 Arthrobacter sp.
-
hexano-6-lactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2 Rhodococcus ruber Phi 2 initial step of the degradation of cyclohexanone to adipic acid epsilon-caprolactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2 Rhodococcus pyridinivorans Phi 1
-
hexano-6-lactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2 Arthrobacter sp. BP2
-
hexano-6-lactone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Q84H88
-
-
Arthrobacter sp. BP2 Q84H88
-
-
Rhodococcus pyridinivorans
-
-
-
Rhodococcus pyridinivorans Phi 1
-
-
-
Rhodococcus ruber F5CEP3
-
-
Rhodococcus ruber Phi 2 F5CEP3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme using His-tag Rhodococcus pyridinivorans
recombinant enzyme using His-tag Arthrobacter sp.
recombinant enzyme using His-tag Rhodococcus ruber

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.57
-
with cyclohexanone as substrate, pH 7.5 Arthrobacter sp.
3.68
-
with cyclohexanone as substrate, pH 7.5 Rhodococcus pyridinivorans
3.75
-
with cyclohexanone as substrate, pH 7.5 Rhodococcus ruber

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexanone + NADPH + O2 initial step of the degradation of cyclohexanone to adipic acid Rhodococcus ruber epsilon-caprolactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2 initial step of the degradation of cyclohexanone to adipic acid Rhodococcus ruber Phi 2 epsilon-caprolactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2
-
Rhodococcus pyridinivorans hexano-6-lactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2
-
Arthrobacter sp. hexano-6-lactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2
-
Rhodococcus ruber hexano-6-lactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2
-
Rhodococcus ruber Phi 2 hexano-6-lactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2
-
Rhodococcus pyridinivorans Phi 1 hexano-6-lactone + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2
-
Arthrobacter sp. BP2 hexano-6-lactone + NADP+ + H2O
-
?
additional information also oxidizes a large variety of cyclic and linear ketones Rhodococcus pyridinivorans ?
-
?
additional information also oxidizes a large variety of cyclic and linear ketones Arthrobacter sp. ?
-
?
additional information also oxidizes a large variety of cyclic and linear ketones Rhodococcus ruber ?
-
?
additional information also oxidizes a large variety of cyclic and linear ketones Rhodococcus ruber Phi 2 ?
-
?
additional information also oxidizes a large variety of cyclic and linear ketones Rhodococcus pyridinivorans Phi 1 ?
-
?
additional information also oxidizes a large variety of cyclic and linear ketones Arthrobacter sp. BP2 ?
-
?

Synonyms

Synonyms Comment Organism
ChnB
-
Rhodococcus pyridinivorans
ChnB
-
Arthrobacter sp.
ChnB
-
Rhodococcus ruber

Cofactor

Cofactor Comment Organism Structure
flavin
-
Rhodococcus pyridinivorans
flavin
-
Arthrobacter sp.
flavin
-
Rhodococcus ruber
NADPH
-
Rhodococcus pyridinivorans
NADPH
-
Arthrobacter sp.
NADPH
-
Rhodococcus ruber