Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli | Rhodococcus pyridinivorans |
expressed as His-tag fusion protein in Escherichia coli | Arthrobacter sp. |
expressed as His-tag fusion protein in Escherichia coli | Rhodococcus ruber |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexanone + NADPH + O2 | Rhodococcus ruber | initial step of the degradation of cyclohexanone to adipic acid | epsilon-caprolactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | Rhodococcus pyridinivorans | - |
hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | Arthrobacter sp. | - |
hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | Rhodococcus ruber Phi 2 | initial step of the degradation of cyclohexanone to adipic acid | epsilon-caprolactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | Rhodococcus pyridinivorans Phi 1 | - |
hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | Arthrobacter sp. BP2 | - |
hexano-6-lactone + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | Q84H88 | - |
- |
Arthrobacter sp. BP2 | Q84H88 | - |
- |
Rhodococcus pyridinivorans | - |
- |
- |
Rhodococcus pyridinivorans Phi 1 | - |
- |
- |
Rhodococcus ruber | F5CEP3 | - |
- |
Rhodococcus ruber Phi 2 | F5CEP3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme using His-tag | Rhodococcus pyridinivorans |
recombinant enzyme using His-tag | Arthrobacter sp. |
recombinant enzyme using His-tag | Rhodococcus ruber |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.57 | - |
with cyclohexanone as substrate, pH 7.5 | Arthrobacter sp. |
3.68 | - |
with cyclohexanone as substrate, pH 7.5 | Rhodococcus pyridinivorans |
3.75 | - |
with cyclohexanone as substrate, pH 7.5 | Rhodococcus ruber |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexanone + NADPH + O2 | initial step of the degradation of cyclohexanone to adipic acid | Rhodococcus ruber | epsilon-caprolactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | initial step of the degradation of cyclohexanone to adipic acid | Rhodococcus ruber Phi 2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Rhodococcus pyridinivorans | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Arthrobacter sp. | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Rhodococcus ruber | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Rhodococcus ruber Phi 2 | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Rhodococcus pyridinivorans Phi 1 | hexano-6-lactone + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Arthrobacter sp. BP2 | hexano-6-lactone + NADP+ + H2O | - |
? | |
additional information | also oxidizes a large variety of cyclic and linear ketones | Rhodococcus pyridinivorans | ? | - |
? | |
additional information | also oxidizes a large variety of cyclic and linear ketones | Arthrobacter sp. | ? | - |
? | |
additional information | also oxidizes a large variety of cyclic and linear ketones | Rhodococcus ruber | ? | - |
? | |
additional information | also oxidizes a large variety of cyclic and linear ketones | Rhodococcus ruber Phi 2 | ? | - |
? | |
additional information | also oxidizes a large variety of cyclic and linear ketones | Rhodococcus pyridinivorans Phi 1 | ? | - |
? | |
additional information | also oxidizes a large variety of cyclic and linear ketones | Arthrobacter sp. BP2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChnB | - |
Rhodococcus pyridinivorans |
ChnB | - |
Arthrobacter sp. |
ChnB | - |
Rhodococcus ruber |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Rhodococcus pyridinivorans | |
flavin | - |
Arthrobacter sp. | |
flavin | - |
Rhodococcus ruber | |
NADPH | - |
Rhodococcus pyridinivorans | |
NADPH | - |
Arthrobacter sp. | |
NADPH | - |
Rhodococcus ruber |