Literature summary for 1.14.13.22 extracted from

Cheesman, M.J.; Byron Kneller, M.; Rettie, A.E.
Critical role of histidine residues in cyclohexanone monooxygenase expression, cofactor binding and catalysis (2003), Chem. Biol. Interact., 146, 157-164.

Search for more literature for 1.14.13.22

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Acinetobacter sp.

Protein Variants

Protein Variants	Comment	Organism
H113Q	only minor effects on activity	Acinetobacter sp.
H162Q	only minor effects on activity	Acinetobacter sp.
H163Q	10fold lower turnover, doubling of Km	Acinetobacter sp.
H200Q	only minor effects on activity	Acinetobacter sp.
H522Q	only minor effects on activity	Acinetobacter sp.
H59Q	not expressed at significant levels	Acinetobacter sp.
H96Q	greatly reduced FAD content, reduced activity	Acinetobacter sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyl dicarbonate	complete inhibition	Acinetobacter sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Acinetobacter sp.	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
61600	-	mass spectrometry	Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cyclohexanone + NADPH + O2	Acinetobacter sp.	-	hexano-6-lactone + NADP+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter sp.	-	NCIB 9871	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.4	-	H163Q mutant enzyme	Acinetobacter sp.
5.4	-	H96Q mutant enzyme	Acinetobacter sp.
15.9	-	wild type enzyme	Acinetobacter sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyclohexanone + NADPH + O2	-	Acinetobacter sp.	hexano-6-lactone + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
cyclohexanone:NADPH:oxygen oxidoreductase (lactone-forming)	-	Acinetobacter sp.

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Acinetobacter sp.
NADPH	-	Acinetobacter sp.

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Release 2023.1 (January 2023)